EXAM 4: QUIZ METHODS: CELLS, PROTEINS

Question 1

true or false: fluorescent light is emitted throughout the specimen being viewed

Answer 1

true

Question 2

early microscopes did not allow clear visualization of cells because they were limited by

Answer 2

resolution

Question 3

the ____ was developed in the late 1920’s by Theodore Svedberg. He originally used it to determine the sedimentation rate of proteins

Answer 3

ultracentrifuge

Question 4

true or false: specimens are always viewed w/o being stained when using a brightfieqld microscope

Answer 4

f

Question 5

what kind of microscope detects electrons passing through a specimen

Answer 5

transmission electron microscope

Question 6

A scientist is examining motile protozoa. He wishes to determine their direction of movement. which microscope is NOT going to be used

Answer 6

electron microscopy

Question 7

____ is a biochemical technique that allows one to separate biological molecules based on size, shape, and/or affinity for specific molecules or functional groups

Answer 7

chromatography

Question 8

what microscope has NOT allowed us to understand cell structure and function

Answer 8

light microscopy

Question 9

you are working on a project that involves the direct 3D observation of DNA molecules. The microscope that would give you the best info at this time would be the

Answer 9

scanning electron microscope

Question 10

what microscope would you use to examine the cytoplasm of a cell for changes that result from viral infection

Answer 10

transmission electron microscopy

Question 11

An ___ is an instrument used to separate subcellular structure and macromolecules on the basis of size, shape, and density. Theodor Svedberg devolved this instrument in Sweden during the period 1925-1930

Answer 11

ultracentrifuge

Question 12

what is an important characteristic for a model organism

Answer 12

widely studied

Question 13

in studying osteoporosis in humans you wish to test a newly designed treatment for efficacy. your best choice for a model organism would be

Answer 13

mus musculus

Question 14

you wish to obtain a pure mitochondria sample from lysed cells. the best way to obtain this sample would be

Answer 14

centrifugation

Question 15

detects electrons deflected from the surface of the specimen

Answer 15

scanning electron microscope

Question 16

____ is the ability to distinguish 2 objects that are close together as separate. when using a light microscope this ability is determine b wavelength

Answer 16

resolution

Question 17

what microscope would you use to determine if a lymphocyte possesses a certain surface protein

Answer 17

fluorescence microscopy

Question 18

what can a electron microscope view

Answer 18

viruses

Question 19

uses a laser to view a single plane of a specimen

Answer 19

confocal microscope

Question 20

scanning electron microscopy is especially suited to

Answer 20

create a sense of depth

Question 21

what microscope would you use to visualize the ribosome of a cell

Answer 21

transmission electron microscope

Question 22

what is not a model organism

Answer 22

homo sapiens

Question 23

light passes directly through specimen

Answer 23

brightfieqld microscope

Question 24

a microtome is used to

Answer 24

slice thin sections of specimens

Question 25

amplifies variation in density

Answer 25

phase-contrast microscope

Question 26

the distance that 2 objects must be apart to be distinguished as separate objects

Answer 26

resolution

Question 27

what microscope would you use to examine the motility of a bacterium

Answer 27

phase/contrast differential-interference-contrast

Question 28

You have a sample of mimivirus which has the largest capsid diameter of all currently known viruses and is hexagon shaped. based on your knowledge of microscopes what would you be able to see/determine about mimiviral structure using each of the following microscopes? simple compound (light) microscope

Answer 28

able to see basic viral shape, especially. if particles are stained

Question 29

You have a sample of mimivirus which has the largest capsid diameter of all currently known viruses and is hexagon shaped. based on your knowledge of microscopes what would you be able to see/determine about mimiviral structure using each of the following microscopes? fluorescent microscope using fluorescently labeled antibodies to a novel capsid protein

Answer 29

will illuminate the outside of the viral particles

Question 30

because of the low penetration power of electrons sales from transmission electron microscopy must be extremely thin. an _____ is able to cut sections as thin as 20 nm

Answer 30

ultramicrotome

Question 31

what microscope would you use to determine the 3D shape of a virus

Answer 31

scanning electron microscope

Question 32

in 1665 ____ used a microscope that he had built to examine thin slices of _____. while examining this material he observed tiny compartments that he called cellulae

Answer 32

• Robert hooke

- cork

Question 33

which type of microscopy has the greatest resovling power

Answer 33

electron

Question 34

shows specific molecule

Answer 34

fluorescence microscope