ETC- Part 1

Question 1

Inner mitochondrial membrane has ____ which makes it have a large surface area

Answer 1

cristae; very impermeable

Question 2

Outer membrane has ____

Answer 2

porins; permeable

Question 3

NADH enters as complex ___ FADH enters as complex ____

Answer 3

1; 2 (succinate to fumerase)

Question 4

+ charges on the ____of the mitochondrial matrix, whereas - charges are on the inside

Answer 4

outside; inside

Question 5

Electrons get transferred from ___ to ___

Answer 5

lower to higher. (higher are the acceptors)

Question 6

Rotenone inhibits transfer of NADH to

Answer 6

Q and Cyt b

Question 7

Antimycin A inhibits transfer of Cyt B to

Answer 7

Cyt C1

Question 8

CN- or CO inhibit transfer of Cyt a to

Answer 8

oxygen

Question 9

What are the enzyme complexes of the ETC

Answer 9

1. NADH dehydrogenase
2. Succinate dehydrogenase
3. Ubiquinone cytochrome c oxidoreductase
4. Cytochrome oxidase

Question 10

Cytochromes consist of protiens + heme ____ ____and have different classes. They accept 1 electron at a time and absorb visible light differently if oxidized or reduced

Answer 10

prosthetic groups

Question 11

What is the non protein component of ETC

Answer 11

ubiquinone; aromatic ring structure with keto groups that give resonance structures

Question 12

ubiquinone is ____soluble and resides in the ___membrane and can accomodate 1 or ____ e- transfers and also carries proton

Answer 12

fat; inner; 2

Question 13

Complex 1, NADH dehydrogenase processes ___e- at a time and converts the e- from NADH to ____and transfers ___protons

Answer 13

2; QH2; 4

Question 14

Complex 2, Succinate Dehydrogenase is fixed in ___membrane and produces ____It doesnt have any transfer of H+ into intermembrane space

Answer 14

inner; FADH2

Question 15

Complex 3, Cytochrome bc1 processes ____ and transfers e- to ______ and pumps protons into intermembrane space. It is loosely associated and floats in the intermembrane space, thus it is considered a ______ _____. It transfers ____protons as well.

Answer 15

QH2YQ; cytochrome c; “step down transformer”; 4

Question 16

cytochrome C has been identified to have an extra _____. It is important in _____.

Answer 16

job; apoptosis (programmed cell death)

Question 17

what is the process of apoptosis in cytochrome c

Answer 17

1. Permeability transition pore complex opens up
2. cytochrome c moves to cytosol
3. Apaf -1 activates
4. Dimerization of Procapsase -9
5. caspase-9 breaks down proteases of cell
6. cell self destructs from inside out

Question 18

complex 4, cytochrome oxidase, takes e- from cytochrome c to oxygen to produce _____. It pumps protons into _____ and is the source of the majority of the cellular _____stress. It transfers ____ protons.

Answer 18

water; IMS; oxidative; 2

Question 19

Complex V, ATP Synthase, converts ATP + Pi to ____. It is very large. The circular motion of F0 driven by proton gradient like a water wheel.

Answer 19

ATP