Enzymes Flashcards
1
Q
Whats a cofactor?
A
A non protein part needed for functionality
2
Q
Whats a coenzyme?
A
An enzyme made from a vitamin
3
Q
What’s a prosthetic group?
A
A cofactor that is covalently bound to the enzyme.
4
Q
What’s an apoenzyme?
A
The protein component of an enzyme containing the prosthetic group
5
Q
How do enzymes catalyse reactions?
A
- Lower activation energy
- Increase rate of spontaneous reactions
- Accelerate movement towards equilibrium.
6
Q
What does Km tell you?
A
The affinity for the enzyme of the substrate
7
Q
How do competitive inhibitors work and what is their affect on Km and Vmax?
A
- Tend to resemble the substrate
- Bind to active site
- Km increases
- Vmax remains the same
8
Q
How do non-competitive inhibitors work and what is their affect on Km and Vmax?
A
- They bind to a non active site on the enzyme.
- The substrate can still bind to the active site so Km remains the same
- Vmax decreases.
9
Q
What is the random model when referring to enzyme-substrate binding?
A
- It doesn’t matter which way the substrates bind the outcome will be the same
10
Q
What is the ordered sequential model?
A
One substrate HAS to bind before the other and the other has to leave first
