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Hematology 1 [LEC] > ENZYMATIC TECHNIQUES > Flashcards

ENZYMATIC TECHNIQUES Flashcards

1
Q

primary granules of granulocytic cells

A

MYELOPEROXIDASE

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2
Q

neutrophils
eosinophils
monocytes
lymphocyte (-)

A

MYELOPEROXIDASE

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3
Q

acute myeloid leukemia (AML) vs. acute lymphoblastic leukemia (ALL)

A

MYELOPEROXIDASE

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4
Q

lysosomes
all hematopoietic cells
T lymphocytes

A

ACID PHOSPHATASE (ACP) without Tartrate
resistance

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5
Q

preliminary identification of T ell subsets of acute lymphoblastic leukemia (ALL)

A

ACID PHOSPHATASE (ACP) without Tartrate
resistance

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6
Q

L-tartaric acid + staining substrate

A

TARTRATE-RESISTANT ACID PHOSPHATASE

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7
Q

hairy cells

A

TARTRATE-RESISTANT ACID PHOSPHATASE

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8
Q

hairy cell leukemia

A

TARTRATE-RESISTANT ACID PHOSPHATASE

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9
Q

diaminobenzidine (DAB) (benzidine substitute)

A

MYELOPEROXIDASE

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10
Q

brown reaction product

A

MYELOPEROXIDASE

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11
Q

copper salts followed by Gill’s modified Papanicolaou stain

A

MYELOPEROXIDASE

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12
Q

intense gray-black granules

A

MYELOPEROXIDASE

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13
Q

neutrophil and monocyte

A

MYELOPEROXIDASE

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14
Q

differentiates cells of lymphoid origin
from granulocytes and their precursors and monocytes

A

MYELOPEROXIDASE

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15
Q

Peripheral blood or bone marrow smears

A

ACID PHOSPHATASE (ACP) without Tartrate
resistance

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

ALKALINE PHOSPHATASE (ALP

ESTERASES

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16
Q

naphthol AS I phosphoric acid and fast garnet GBC salt.

A

ACID PHOSPHATASE (ACP) without Tartrate
resistance

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17
Q

enzymatic hydrolysis

A

ACID PHOSPHATASE (ACP) without Tartrate
resistance

Erastase

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18
Q

insoluble maroon dye

A

ACID PHOSPHATASE (ACP) without Tartrate
resistance

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19
Q

cellular sites

A

ACID PHOSPHATASE (ACP) without Tartrate
resistance

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20
Q

L- tartrate–containing substrate

A

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

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21
Q

Cells containing tartaric acid–sensitive acid phosphatase do not exhibit any dye deposits

A

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

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22
Q

mononuclear cells containing tartaric acid–resistant acid phosphatase are not affected by such treatment

A

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

23
Q

leukocytes (+)
lymphocytes ()

A

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

24
Q

tartrate resistant

A

hairy cell leukemia, Sézary syndrome, and some T-cell acute lymphoblastic leukemias
are

25
Q

ACP IsoE 5

A

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

26
Q

alkaline-dye solution

A

ALKALINE PHOSPHATASE (ALP

27
Q

alkaline-dye solution

A

ALKALINE PHOSPHATASE (ALP)

28
Q

naphthol AS-MX phosphate

A

ALKALINE PHOSPHATASE (ALP)

29
Q

‘fast blue RR salt or fast violet B salt

A

ALKALINE PHOSPHATASE (ALP)

30
Q

diazonium salt

A

ALKALINE PHOSPHATASE (ALP)
ESTERASES

31
Q

insoluble, visible pigment

A

ALKALINE PHOSPHATASE (ALP)

32
Q

Leukocyte alkaline phosphatase (LAP) activity

A

ALKALINE PHOSPHATASE (ALP)

33
Q

leukemoid reactions and chronic granulocytic (myelogenous) leukemia

A

ALKALINE PHOSPHATASE (ALP)

34
Q

touch preparations

A

ESTERASES

35
Q

a Naphthol compound

A

ESTERASES

36
Q

Naphthol AS-D chloroacetate

A

ESTERASES

37
Q

alphanaphthyl acetate or alpha-naphthyl butyrate

A
38
Q

stable diazonium salt

A

ESTERASES

39
Q

highly colored deposits

A

ESTERASES

40
Q

granulocytic vs. monocytic

A

ESTERASES

41
Q

differentiation of leukemias

A

ESTERASES

42
Q

• identify the cells of the granulocytic series. It does not stain lymphocytes and monocytes

A

Specific esterase (Naphthol AS-D chloroacetate)

43
Q

• It can even be used to identify granulocytes in formalin fixed tissue

A

Specific esterase (Naphthol AS-D chloroacetate)

44
Q

• In this procedure, blood or bone marrow smears are incubated with naphthol AS-D chloroacetate in the presence of a stable diazonium salt

A

Specific esterase (Naphthol AS-D chloroacetate)

45
Q

• Naphthol compounds are coupled with diazonium salt, forming highly colored deposits at the sites of enzyme activity

A

Specific esterase (Naphthol AS-D chloroacetate)

46
Q

• seen in monocytes (see Figure 5

A

Non-specific esterase (Alpha-naphthyl acetate esterase

47
Q

• Both α-naphthyl butyrate or α-naphthyl acetate can be

A

Non-specific esterase (Alpha-naphthyl acetate esterase

48
Q

The cells of granulocytic series are negative with this stain

A

Non-specific esterase (Alpha-naphthyl acetate esterase

49
Q

The α -naphthyl butyrate stain is more specific, while the αnaphthyl acetate stain is
more sensitive

A

Non-specific esterase (Alpha-naphthyl acetate esterase

50
Q

In a suspected myeloid leukemia, dual esterase stains can be used as they can
simultaneously classify the blasts as either myeloblasts or monoblasts

A

Non-specific esterase (Alpha-naphthyl acetate esterase

51
Q

To differentiate positive reacting cells conclusively from monocytes, sodium fluoride is
incorporated with the incubation system

A

Non-specific esterase (Alpha-naphthyl acetate esterase

52
Q

The monocyte enzyme is inactivated in the presence of this compound

A

Non-specific esterase (Alpha-naphthyl acetate esterase

53
Q

Monocytic non-specific esterase is Fluoride sensitive.

A

Non-specific esterase (Alpha-naphthyl acetate esterase

Hematology 1 [LEC] (25 decks)

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