EBV, CMV, and Viral Latency and Cancer

Question 1

EBV is latent in what cells and as what type of genome

Answer 1

myeloid B cells through CD21; episomal DNA in the nucleus of the infected B-cells

Question 2

acute lytic infection

Answer 2

3-14 days, fever, fatigue, lymphadenopathy, headache, nausea, diarrhea, influenza, e bola, or norovirus

Question 3

chronic lytic infection

Answer 3

virus continues to replicate

Question 4

latent viral infection

Answer 4

virus does not continue to develop

Question 5

would a sensitive test for viral particles be helpful in determining a virus in latency or in chronic infection?

Answer 5

chronic infection

Question 6

chronic focal infection

Answer 6

cells infected with viral particle infect other cells by lysing

Question 7

chronic diffuse infection

Answer 7

cells do not lyse but continue to replicate new cells

Question 8

hepatitis is an example of

Answer 8

chronic lytic infection

Question 9

both chronic and latent infections can be _____ latent; the difference is in the molecular state of the virus

Answer 9

clinically

Question 10

provirus

Answer 10

integrated into the host genome during latency

Question 11

episome

Answer 11

integrated into extrachromosomal DNA during latency

Question 12

during latency both RNA and DNA viruses are stored as _____ genomes

Answer 12

DNA

Question 13

EBV was the first

Answer 13

virus to be discovered to have a connection to cancer

Question 14

what type of shape is EBV

Answer 14

icosahedral core

Question 15

does EBV have a lipid envelop

Answer 15

yes

Question 16

where does EBV get its lipid envelop

Answer 16

the host

Question 17

herpesvirus genomes are

Answer 17

linear ds DNA

Question 18

How is EBV transmitted

Answer 18

saliva

Question 19

how common is EBV worldwide

Answer 19

90% of adults have the antibodies

Question 20

route of transmssion

Answer 20

1. infects epithelial cells
2. replicates
3. enters the blood stream
4. attacks B cells via CD21 complement receptor
5. T cells keep the virus in latent state

Question 21

early clinical symptoms of EBV and then usually develop into

Answer 21

asymptomatic; mononucleosis

Question 22

clinical signs and symptoms of infectious mononucleosis

Answer 22

fatigue, fever, sore throat, lymphadenopathy

Question 23

what does mononucleosis mean

Answer 23

increased lymphocytes that are atypical

Question 24

EBV diagnosis

Answer 24

clinically diagnosed, monospot test but increased false positives and false negatives; uses heterophile antibody

Question 25

when can you have a positive monospot test

Answer 25

2weeks of illness

Question 26

EBV specific antibodies

Answer 26

IgM early and IgG later

Question 27

Is acyclovir effective against CMV or EBV?

Answer 27

no

Question 28

Is there a vaccine for EBV or CMV?

Answer 28

no

Question 29

EBV associated diseases?

Answer 29

1. Burkitts lymphoma
2. Hairy leukoplakia
3. severe life threatening mono in children with x-linked lymphoproliferative syndrome

Question 30

EBV in latency is producing what IL resulting in?

Answer 30

IL-10, uncontrolled proliferation

Question 31

CMV establishes latency in

Answer 31

many types of cells

Question 32

CMV is transmitted via

Answer 32

saliva, genital secretions, trasfusions and transplacentally

Question 33

what is the most common cause of congenital abnormalities in the US?

Answer 33

CMV

Question 34

what do you have to do before a transplant in regard to CMV?

Answer 34

sero match for CMV

Question 35

what is seen on a histology stain with CMV?

Answer 35

giant cell formation with intranuclear inclusions that looks like an alien

Question 36

primary infection in adults and kids are

Answer 36

asymptomatic

Question 37

clinical findings with CMV

Answer 37

blueberry muffin lesions

Question 38

what are the results for heterophile antibody for EBV and CMV?

Answer 38

positive and negative respectively

Question 39

CMV treatment

Answer 39

ganciclovir and valgancyclovir

Question 40

ganciclovir and valgancyclovir can treat

Answer 40

pneumonia and retinitis symptoms

Question 41

why is ganciclovir and valgancyclovir effective and acyclovir not?

Answer 41

activated by cellular kinases and not HSV kinases