assays for hemostasis

Question 1

megakaryoctyes

Answer 1

develop from hemopoietic stem cells that reside in bone morrow

Question 2

Meg-CFC (megokaryocyte colony simulating factor) then begin ____ in which the DNA replication continues but neither the nucleus nor the cell undergoes division this produces a ________ cell

Answer 2

endomitosis; polyploid

Question 3

C-Mpl

Answer 3

TPO receptor on megokaryocytes and platelets; when TPO binds it increase platelet synthesis

Question 4

TPO

Answer 4

thrombopoietin; stimulates platelet synthesis, made in the liver; static

Question 5

phospholipid scaffold is important for

Answer 5

secondary coagulation factors

Question 6

alpha2beta1

Answer 6

GP2GP3

binds collagen

Question 7

alpha2bbeta3

Answer 7

GPIIbGPIIIa

binds fibrinogen

Question 8

GP Ib- IX-V

Answer 8

binds von willebrand

Question 9

GP VI

Answer 9

activates platelets by binding collagen

Question 10

platelet plug formation (4)

Answer 10

1. Adhesion
2. Activation
3. Aggregation
4. Secretion

Question 11

adhesion

Answer 11

GP Ib- IX-V binding to VWF

Question 12

activation

Answer 12

platelets become activated and secrete GP IIB-IIIA

Question 13

aggregation

Answer 13

GP IIb IIIa activates fibrinogen

Question 14

secretion

Answer 14

platelets release granular contents and potentiate clotting

Question 15

qualitative platelet disorders can be

Answer 15

congenital or acquired

Question 16

acquired platelet disroders

Answer 16

1. NSAIDS and aspirin
2. herbs
3. myeloproliferative disease
4. uremia

Question 17

congenital

Answer 17

1. Bernards- GP Ib IX-V deficiency- lack of platelet adhesion
2. Glanzmanns thrombaesthenia- GP IIb-IIIa deficiency- lack of aggregation

Question 18

platelet dysfunction symptoms

Answer 18

• mucocutaneous bleeding

- same as primary hemostasis- epistaxis, gum bleeding, bruising, heavy menses, petechiae

Question 19

how to quantify platelet disorders

Answer 19

PFA platelet function analyzer 100; bleeding time

Question 20

what keeps platelets from sticking to normal endothelium?

Answer 20

• NO
• Prostacyclin
• ecto-ADPase- chews up ADP to prevent platelet activation

Question 21

collagen

Answer 21

GP Ib IX-V

GP VI

Question 22

thrombin

Answer 22

• most potent platelet agonist

- PAR-1 receptor bind thrombin

Question 23

thromboxane

Answer 23

COX 1 activation leads to production of thromboxane

- thromboxane receptor is GPCR

Question 24

ADP

Answer 24

• secreted by lysed RBC and activated platelets
• PY12 and PY1 receptors
• GPCR

Question 25

when the spleen gets enlarged platelets like to go there to hang out instead of being in the circulation. The platelet count drops. What happens to TPO levels?

Answer 25

remain constant because spleen is technically still in the spleen

Question 26

proplatelets

Answer 26

long branching process extended by mature megokaryocytes into the sinusoidal blood vessels of the bone marrow

Question 27

vitamin K is a water/fat soluble vitamin

Answer 27

fat

Question 28

vitamin K is used to make zymogens/cofactor coagulation factors

Answer 28

zymogen

Question 29

vitamin K pro- coagulation factors

Answer 29

2, 7, 9, 10

Question 30

vitamin K anticoagulation factors

Answer 30

protein C and S

Question 31

what four effects could cause prolonged PT?

Answer 31

1. vitamin K deficiency- lack of green leafy vegetables
2. vitamin K inhibitors- warfarin
3. liver disease
4. all factors that cause prolonged TT
5. DIC