DNA Replication Flashcards

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1
Q

What is the term for the type of replication where half of the strands in each new DNA molecule come from the original DNA?

A

Semi-conservative replication

This method of replication ensures genetic continuity.

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2
Q

What is the role of DNA helicase during DNA replication?

A

DNA helicase breaks hydrogen bonds between bases to unwind the DNA

This unwinding is essential for the separation of the two DNA strands.

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3
Q

What is the function of DNA polymerase in DNA replication?

A

Catalyzes condensation reactions to join adjacent nucleotides

This enzyme is crucial for synthesizing the new DNA strand.

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4
Q

What are the components of a nucleotide in DNA?

A
  • Deoxyribose (pentose sugar)
  • Nitrogenous bases (purines and pyrimidines)
  • Phosphate group

The sequence of these components encodes genetic information.

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5
Q

What base pairs with adenine in DNA?

A

Thymine

This is part of the base pairing rules in DNA structure.

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6
Q

What is the structure of DNA?

A

Double helix formed by two strands held by hydrogen bonds

The strands are antiparallel, meaning they run in opposite directions.

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7
Q

Which of the following is a type of DNA mutation: Substitution, Duplication, or Inversion?

A
  • Substitution
  • Deletion
  • Addition
  • Duplication
  • Inversion
  • Translocation

These mutations can affect genetic information in various ways.

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8
Q

What happens during condensation reactions in DNA replication?

A

Adjacent nucleotides are joined together

This process is essential for forming the backbone of the DNA strand.

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9
Q

Fill in the blank: DNA replication occurs before _______.

A

cell division

This ensures that each new cell receives a complete set of genetic information.

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10
Q

True or False: DNA is positively charged because of the phosphate.

A

False

DNA is negatively charged due to the phosphate groups in its backbone.

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11
Q

What is the significance of gene mutations during DNA replication?

A

They can lead to changes in the genome

While some mutations may be benign, others can lead to diseases.

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12
Q

How many genetically identical cells are produced by mitosis?

A

2

Mitosis is a process of cell division that results in two identical daughter cells.

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13
Q

What do shorter DNA fragments do in gel electrophoresis?

A

Move faster than longer DNA fragments

This principle is used to separate DNA fragments based on size.

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14
Q

What is a stem cell?

A

A specialized cell capable of differentiation

Stem cells can develop into various cell types in the body.

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15
Q

What is the purpose of the participation assessment in the lecture?

A

To ensure engagement and understanding of the material covered

It’s required to complete every question of the quiz to earn participation marks.

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16
Q

What is the original strand of DNA used for during replication?

A

It serves as a template for synthesizing new strands

This ensures that the new DNA is complementary to the original.

17
Q

In DNA, which bases are purines?

A
  • Adenine
  • Guanine

Purines are larger nitrogenous bases compared to pyrimidines.

18
Q

What are the pyrimidines found in DNA?

A
  • Cytosine
  • Thymine

Pyrimidines are smaller nitrogenous bases that pair with purines.