DL - diagnostic tests for immune-mediated disease Flashcards
1
Q
On a Coombs’ test, what does a shield result suggest?
A
That autoagglutination has occured (a button suggest not autoagglutination has occured)
2
Q
Use - Coombs’ test
A
for suspect IMHA
3
Q
What is the basis of the ANA test?
A
Immunoflourescence of anti-canine IgG Ab labelled with fluorescent tag (fluorescence = positive result)
4
Q
What is Allercept assay for?
A
Flea-allergy dermatitis
5
Q
What is the concept of the Allercept assay for FAD?
A
Ag on test well, serum containing IgE added that can bind to Ag, Mast cells expresssing Fc epsilon receptors are added, then substrate is added. Colour change if mast cells bind to Ab bound to Ag.
6
Q
What is the concept of the thyroglobulin autoantibdies in canine hypothyroidism?
A
similar to allercept assay for FAD
