Deck 9

Question 1

12) Which of the following statements is TRUE:

a. Lack of generation of bactericidal agents is related with defect in phagocytic function
b. Lack of receptor for chemotactic factor is related with defect in phagocytic function
c. Lack of adhesion molecules is related with defect in phagocytic function
d. All of the above is true

Answer 1

D

Question 2

Which of the following components is for IFA:

a. Antibody labelled with fluorochrome
b. Antibody labelled with peroxidase
c. Antibody labelled with alkaline phosphatase
d. Antibody labelled with radioisotope

Answer 2

Antibody labelled with fluorochrome

Question 3

The direct IFA is used for:

a. Determination of antigen in a cell or tissue by direct labelling with antibodies labelled with
FITC
b. Determination of antigen in a cell or tissue by indirect labelling with antibodies labelled with
FITC
c. Quantitative determination of antibody

Answer 3

Determination of antigen in a cell or tissue by direct labelling with antibodies labelled with FITC

Question 4

The indirect IFA is most commonly used for:

a. Testing of autoimmune disorders
b. Determination of viral antigen in tissue
c. Determination of bacterial and viral antigen in tissue
d. Determination of CD antigen

Answer 4

Testing of autoimmune disorders

Question 5

To develop a test to screen circulating anti-brucella antibodies by immunofluorescence, you need all of the following EXCEPT:

a. A fluorescence microscope
b. A positive serum control, know to contain anti-brucella antibodies
c. A suspension of formalin-killed Brucella melitensis
d. Fluorescein-labelled anti-bovine IgG
e. Isolated patient’s IgG labelled with a fluorescein compound

Answer 5

Isolated patient’s IgG labelled with a fluorescein compound

Question 6

FACS is used for:

a. Analysis and sort a mixture of the cells based on their differential ability to bind enzymelabelled
ATB
b. Reading of absorbance
c. Determination of CD antigen on the cell surface
d. Analysis and sort a mixture of the cells based on their differential ability to bind
fluorescence-dye labelled ATB

Answer 6

Analysis and sort a mixture of the cells based on their differential ability to bind fluorescence-dye labelled ATB

Question 7

The essential step for western blotting diagnosis of BSE is:

a. Electrophoresis of sample
b. Treatment of the sample with proteinase K
c. Transfer of sample to nitrocellulose filter (NF)
d. Treatment of NF with Proteinase K

Answer 7

Treatment of the sample with proteinase K

Question 8

Serological reaction is:

Answer 8

• Serology is the scientific study of blood serum and other body fluids
• Serological reaction is Ag + Ab → AgAb, antigen binds to the antibody

Question 9

The titer of an antibody is:

A) The first dilution of an antiserum that react with antigen in serological reactions.
B) The highest dilution of an antiserum that still react with antigen in serological
reactions (X)
C) The lowest dilution of an antiserum that still react with antigen in serological reactions.
D) Last negative dilution of antiserum

Answer 9

The highest dilution of an antiserum that still react with antigen in serological reactions (X)

Question 10

Serum is obtained after:

A) Centrifugation of blood.
B) Centrifugation of clotted blood (X)
C) After 1h incubation of unclotted blood at 37grader
D) After 1h incubation of clotted blood at 37grader

Answer 10

Centrifugation of clotted blood (X)