Deck 5

Question 1

The titre of antibody is

Answer 1

The highest dilution of an antiserum that still react with antigen in serological reactions

Question 2

The difference between agglutination and precipitation is in

Answer 2

Size, solubility and location of antigen

Question 3

The main deficiency of quantitative assays based on precipitation is

Answer 3

Lack of sensitivity

Question 4

Which of the following immune-diffusion assays is for quantitative determination of antigen

Answer 4

Single radial immune-diffusion

Question 5

Ouchterlony’s precipitation is used

Answer 5

Only for determination of antigen or antibody presence

Question 6

Antibody is added to agarose used for

Answer 6

Single radial immunodiffusion

Question 7

Result of immune-precipitation in the gel is visible after at least

Answer 7

24 h incubation at room temperature

Question 8

The precipitation band has

Answer 8

White colour

Question 9

Draw the scheme of precipitin lines for
(check notes this isn’t a good version)

Answer 9

Reaction of identity Reaction on partial identity Reaction of non-identity
Agl Agl Agl Agl,2 Agl Ag2
O O O O O O

 O				   O					    O
       Anti – 1				Anti 1,2					Anti 1,2

Question 10

Which of the following immunoelectrophoresis is for quantitative determination of antigen

Answer 10

Rocket immunoelectrophoresis