Deck 5

Question 1

The titre of antibody is:

a. The first dilution of an antiserum that react with antigen in serological reactions
b. The highest dilution of an antiserum that still react with antigen in serological reactions
c. The lowest dilution of an antiserum that still react with antigen in serological reaction
d. Last negative dilution of antiserum

Answer 1

The highest dilution of an antiserum that still react with antigen in serological reactions

Question 2

The difference between agglutination and precipitation is in:

a. Size, solubility and location of antigen
b. Size of antibody
c. Solubility and concentration of antigen
d. Concentration of antibody

Answer 2

Size, solubility and location of antigen

Question 3

The main deficiency of quantitative assays based on precipitation is:

a. Difficult in obtaining reagents
b. Lack of sensitivity
c. Short shelf-life of reagent
d. High sensitivity

Answer 3

Lack of sensitivity

Question 4

Which of the following immune-diffusion assays is for quantitative determination of antigen:

a. Oudin’s tube precipitation
b. Single radial immune-diffusion
c. Double radial immune-diffusion (Ouchterlony’s precipitation)
d. Ascoly thermoprecipitation

Answer 4

Single radial immune-diffusion

Question 5

Ouchterlony’s precipitation is used:

a. Only for determination of antigen or antibody presence
b. Only for determination of antigen concentration
c. Only for determination of antibody concentration
d. Only for determination of antigen presence

Answer 5

Only for determination of antigen or antibody presence

Question 6

Antibody is added to agarose used for:

a. Single radial immunodiffusion
b. Double radial immunodiffusion (Ouchterlony’s precipitation)
c. Classical immune-electrophoresis
d. Counter immune-electrophoresis

Answer 6

Single radial immunodiffusion

Question 7

Result of immune-precipitation in the gel is visible after at least:

a. 4 h incubation at 37 °C
b. 24 h incubation at 4 °C
c. 72 h incubation at room temperature
d. 24 h incubation at room temperature

Answer 7

24 h incubation at 4*C

Question 8

The precipitation band has

Answer 8

White colour

Question 9

Draw the scheme of precipitin lines for
(check notes this isn’t a good version)

Answer 9

Reaction of identity Reaction on partial identity Reaction of non-identity
Agl Agl Agl Agl,2 Agl Ag2
O O O O O O

 O				   O					    O
       Anti – 1				Anti 1,2					Anti 1,2

Question 10

Which of the following immunoelectrophoresis is for quantitative determination of antigen:

a. Classical immunoelectrophoresis
b. Rocket immunoelectrophoresis
c. Counter immunoelectrophoresis
d. Two dimensional (crossed) immunoelectrophoresis

Answer 10

Rocket immunoelectrophoresis