Deck 10

Question 1

Visible clumping – agglutination. Agglutination is used to determine presence of antibodies/antigens
Agglutination reaction is a reaction between

Answer 1

Corpuscular Ag and polyclonal Ab

Question 2

Which of the following immunodiffusion assays is for quantitative determination of Ag?

Answer 2

Single radial immunodiffusion

Question 3

What is a conjugate?

Answer 3

Ab labelled with enzyme

Question 4

Draw the scheme of blocking ELISA for detection of Ab
(NOTES)

Answer 4

check notes

Question 5

Which component is used for IFA? (Immunofluorescent assay)

Answer 5

Ab labelled with fluorochrome

Question 6

Polymorponuclear cells are:

Answer 6

Neutrophils, basophils, eosinophils

Question 7

Size of the cells in flow cytometer is detected by:

Answer 7

Forward scatter

Question 8

For determination of number of leukocytes the blood is

Answer 8

Diluted in Türk solution (1:20)

Question 9

Calculate the phagocytic activity if from 100 counted phagocytes 65 cells have ingested material:

Answer 9

% PHA = (65 / 100) * 100 = 65%

Question 10

What is a main purpose of respiratory burst in phagocytes?

Answer 10

Creation of oxidative radicals which destroy ingested material