Deck 10 Flashcards
Visible clumping – agglutination. Agglutination is used to determine presence of antibodies/antigens
Agglutination reaction is a reaction between:
A) Corpuscular antigen and monoclonal antibody
B) Soluble antigen and monoclonal Ab
C) Corpuscular Ag and polyclonal Ab (X)
D) Soluble Ag and polyclonal Ab
Corpuscular Ag and polyclonal Ab
Which of the following immunodiffusion assays is for quantitative determination of Ag?
A) Oudin’s tube precipitation
B) Single radial immunodiffusion (X)
C) Double radial immunodiffusion (ouchterlony’s precipitation)
D) Ascoli termo precipitation
Single radial immunodiffusion
What is a conjugate?
Ab labelled with enzyme
Draw the scheme of blocking ELISA for detection of Ab
(NOTES)
check notes
Which component is used for IFA? (Immunofluorescent assay):
A) Ab labelled with fluorochrome (X)
B) Ab labelled with peroxidase
C) Ab labelled with alkaline phosphatase
D) Ab labelled with radioisotope
Ab labelled with fluorochrome
Polymorponuclear cells are:
A) Monocytes, lymphocytes, NK cells
B) Neutrophils, basophils, eosinophils (X)
C) Basophils, mast cells, lymphocytes
D) macrophages, eosinophils, neutrophils
Neutrophils, basophils, eosinophils
Size of the cells in flow cytometer is detected by:
Forward scatter
For determination of number of leukocytes the blood is
Diluted in Türk solution (1:20)
Calculate the phagocytic activity if from 100 counted phagocytes 65 cells have ingested material:
% PHA = (65 / 100) * 100 = 65%
What is a main purpose of respiratory burst in phagocytes?
A) Creation of oxidative products for increase of metabolic activity of …
B) Creation of oxidative radicals which destroy ingested material (X)
C) Oxidation of membrane bound phospholipids
D) Activation of oxidases
Creation of oxidative radicals which destroy ingested material
