Deck 3

Question 1

A negative reaction in a tube agglutination test is

Answer 1

white sediment (with the shape of a dot) on the bottom and a turbit supernatant

Question 2

In an indirect ELISA for testing of antibody, the intensity of colour measured after adding the substrate in the final step is directly proportional to the concentration of:

Answer 2

antibodies in the patient serum

Question 3

The speed of the movement of molecules during electrophoresis is dependent on

Answer 3

molecular mass

Question 4

Agarose gel is prepared by

Answer 4

boiling of agarose suspension

Question 5

To create a calibration curve in immunodiffusion tests, we need to know

Answer 5

antigen concentration and diameter of precipitation rings

Question 6

Double radial immunodiffusion with antibodies against antigens A and B produced these precipitation lines

Answer 6

If there is antigen B in well 3, there is antigen [B] in well 2, antigen [A] in well 1, and antigen [B] in well 6.
(drawing)

Question 7

To determine a titer of antibodies is used a ……………. dilution of serum.

Answer 7

two fold

Question 8

In ELISA tests substrate is used for

Answer 8

visualisation of the reaction

Question 9

Antibodies are added into the agarose for

Answer 9

single radial immunodiffusion

Question 10

Polyacrylamide (PAA) gel is prepared by polymerisation after

Answer 10

pouring of a PAA solution into a cassette created by two glass plates