Deck 3

Question 1

A negative reaction in a tube agglutination test is:

a. white sediment (with the shape of a dot) on the bottom and a turbit supernatant.
b. white sediment (with the shape of a dot) on the bottom and a clear supernatant.
c. no presence of sediment on the bottom.
d. the only turbidity is formed in the solution.

Answer 1

white sediment (with the shape of a dot) on the bottom and a turbit supernatant

Question 2

In an indirect ELISA for testing of antibody, the intensity of colour measured after adding the substrate in the final step is directly proportional to the concentration of:

a. the substrate.
b. antigen in the solid phase.
c. enzyme-labelled antibody.
d. antibodies in the patient serum.

Answer 2

antibodies in the patient serum

Question 3

The speed of the movement of molecules during electrophoresis is dependent on:

a. total electric charge.
b. molecular mass.
c. chemical composition.
d. isoelectric point.

Answer 3

total electric charge

Question 4

Agarose gel is prepared by:

a. freezing of agarose suspension.
b. boiling of agarose suspension.
c. incubation of agarose suspension in the refrigerator only.
d. incubation of agarose suspension at room temperature only

Answer 4

boiling of agarose suspension

Question 5

To create a calibration curve in immunodiffusion tests, we need to know:

a. both the concentration of antigen and antibodies.
b. only the concentration of antibodies.
c. antigen concentration and diameter of precipitation rings.
d. oonly the diameter of the precipitation rings.

Answer 5

antigen concentration and diameter of precipitation rings

Question 6

Double radial immunodiffusion with antibodies against antigens A and B produced these precipitation lines

Answer 6

If there is antigen B in well 3, there is antigen [B] in well 2, antigen [A] in well 1, and antigen [B] in well 6.
(drawing)

Question 7

To determine a titer of antibodies is used a ……………. dilution of serum.

Answer 7

two fold

Question 8

In ELISA tests substrate is used for:

a. visualization of the reaction.
b. washing.
c. binding of antigen to the solid phase.
d. binding of antigens and antibodies.

Answer 8

visualisation of the reaction

Question 9

Antibodies are added into the agarose for:

a. double radial immunodiffusion.
b. single radial immunodiffusion.
c. Western blot.
d. immunofixation.

Answer 9

single radial immunodiffusion

Question 10

Polyacrylamide (PAA) gel is prepared by polymerisation after:

a. boiling of a PAA solution.
b. boiling of a PAA solution and pouring it into a glass cassette.
c. pouring of a PAA solution on a glass plate.
d. pouring of a PAA solution into a cassette created by two glass plates.

Answer 10

pouring of a PAA solution into a cassette created by two glass plates