Deck 2

Question 1

Which of the following agglutination assays is for quantitative determination of antibodies?

a. Haemagglutination inhibition test.
b. Slide agglutination.
c. Tube agglutination.
d. Latex agglutination.

Answer 1

• Tube agglutination

Question 2

Prozone is:

a. ability to cause agglutination in excess of antibodies.
b. inability to cause agglutination in excess of antibodies.
c. inability to cause agglutination in a lack of antibodies.
d. absence of agglutination.

Answer 2

inability to cause agglutination in excess of antibodies

Question 3

Protein A can bind to:

a. the Fab region of the antibody.
b. biotin.
c. the Fc region of the antibody.
d. streptavidin

Answer 3

the Fc region of the antibody

Question 4

After serum electrophoresis, immunoglobulins are present in the fraction of:

a. gamma globulins.
b. beta globulins.
c. alpha globulins.
d. albumin.

Answer 4

gamma globulins

Question 5

The result of a Western blot is evaluated as a change of colour:

a. on the NC membrane.
b. of the solution.
c. on the gel.
d. in the well.

Answer 5

on the NC membrane

Question 6

SDS (sodium dodecyl sulphate) is used for the preparation of samples for PAGE because of:

a. gives the same positive charge to all proteins.
b. facilitates the movement of proteins in the gel.
c. breaks down protein to subunits.
d. gives the same negative charge to all proteins.

Answer 6

gives the same negative charge to all proteins

Question 7

Serum inactivation is used to:

a. inactivate non-specific antibodies.
b. functionally inactivate specific antibodies.
c. inactivate acute-phase proteins.
d. functionally inactivates the complement system

Answer 7

functionally inactivates the complement system

Question 8

In the sandwich ELISA they have:

a. labelled antibodies have the same specificity as solid-phase bound antibodies.
b. labelled antibodies with different specificity than solid-phase bound antibodies.
c. antibodies with the same specificity as labelled antibodies bound to the solid
phase.
d. antibodies with the same specificity as solid-phase bound antibodies

Answer 8

labelled antibodies with different specificity than solid-phase bound antibodies.

Question 9

Which of the following immunoelectrophoresis is for determination of gammopathies in blood serum?

a. Rocket immunoelectrophoresis.
b. Immunofixation.
c. Classical immunoelectrophoresis.
d. Counter immunoelectrophoresis.

Answer 9

Immunofixation

Question 10

Fill in the blanks in the following statements with the most appropriate expression:

1.Whole blood is used to determine immunoglobulin concentrations using a [?] test

2. Blood serum is used to determine immunoglobulin concentrations using a [?] test

Answer 10

• Whole blood is used to determine immunoglobulin concentrations using a [glutaraldehyde] test.
• Blood serum is used to determine immunoglobulin concentrations using a [zinc sulphate ] test.