Deck 1

Question 1

In a blocking ELISA for testing of antibody:

a. enzyme labelled antibody is added after the first incubation with the serum containing antibody.
b. enzyme labelled antigen is first incubated with an immobilised antibody.
c. enzyme labelled antibody is added together with the sample.
d. enzyme labelled antibody is immobilised in a solid phase.

Answer 1

enzyme labelled antibody is added after the first incubation with the serum- containing antibody.

Question 2

The zone of equivalence is a place, where:

a. is an abundance of antigenes.
b. is an abundance of antibodies.
c. the concentration of antigen is in balance with the concentration of antibodies.
d. the concentration of antigen is not in balance with the concentration of
antibodies.

Answer 2

the concentration of antigen is in balance with the concentration of antibodies

Question 3

The wavelength used for spectrometric analysis of ELISA tests is dependent on the used:

a. enzyme.
b. substrate.
c. conjugate.
d. antigen.

Answer 3

substrate

Question 4

The preparation of hybridomas requires:

a. Plasma cells from the liver of immunized mice and T-lymphocytes from the blood
of healthy mice.
b. B-lymphocytes from the spleen of immunized mouse and myeloma cells.
c. B-lymphocytes from the liver of immunized mice and T-lymphocytes from the
spleen of healthy mice.
d. Plasma cells from the blood of immunized mice and tumor T cells from the
spleen of healthy mice.

Answer 4

B-lymphocytes from the spleen of immunized mouse and myeloma cells

Question 5

The essential step for Western blot diagnosis of BSE is:

a. treatment of the nitrocellulose (NC) membrane with Proteinase K.
b. treatment of the sample with Proteinase K.
c. electrophoresis of the sample.
d. transfer of the sample to the nitrocellulose (NC) membrane.

Answer 5

treatment of the sample with Proteinase K

Question 6

Gammopathies are diseases characterized by:

a. overproduction of acute-phase proteins.
b. overproduction of defective immunoglobulins.
c. overproduction of complement proteinsd.
decreased production of defective immunoglobulins.

Answer 6

overproduction of defective immunoglobulins

Question 7

In electrophoresis individual molecules are separated depending on their:

a. chemical composition.
b. molecular mass.
c. total electric charge.
d. total electric charge and molecular mass

Answer 7

total electric charge and molecular mass

Question 8

A positive reaction in a tube agglutination test is:

a. the only turbidity is formed in the solution.
b. white sediment (with the shape of a dot) on the bottom and a turbit supernatant.
c. white sediment (with the shape of a dot) on the bottom and a clear supernatant.
d. no presence of sediment on the bottom.

Answer 8

white sediment (with the shape of a dot) on the bottom and a clear supernatant

Question 9

The precipitin band has/is

Answer 9

a white colour

Question 10

Fill in the blanks in the following statements with the most appropriate expression

Answer 10

Blood [serum] is prepared by centrifugation of the clotted blood.
Blood [plasma] is prepared by centrifugation of whole blood