Deck 1

Question 1

In a blocking ELISA for testing of antibody

Answer 1

enzyme labelled antibody is added after the first incubation with the serum- containing antibody.

Question 2

The zone of equivalence is a place, where

Answer 2

the concentration of antigen is in balance with the concentration of antibodies

Question 3

The wavelength used for spectrometric analysis of ELISA tests is dependent on the used

Answer 3

substrate

Question 4

The preparation of hybridomas requires

Answer 4

B-lymphocytes from the spleen of immunized mouse and myeloma cells

Question 5

The essential step for Western blot diagnosis of BSE is

Answer 5

treatment of the sample with Proteinase K

Question 6

Gammopathies are diseases characterized by

Answer 6

overproduction of defective immunoglobulins

Question 7

In electrophoresis individual molecules are separated depending on their

Answer 7

total electric charge and molecular mass

Question 8

A positive reaction in a tube agglutination test is

Answer 8

white sediment (with the shape of a dot) on the bottom and a clear supernatant

Question 9

The precipitin band has/is

Answer 9

a white colour

Question 10

Fill in the blanks in the following statements with the most appropriate expression

Answer 10

Blood [serum] is prepared by centrifugation of the clotted blood.
Blood [plasma] is prepared by centrifugation of whole blood