Deck 11 Flashcards

1
Q

To determine a titer of antibodies is used a … dilution of serum:

a. Any
b. Twofold (X)
c. Fivefold
d. Tenfold

A

Two fold

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2
Q

In latex agglutination:

a. Antigen is a latex particle
b. Latex particle is carrier of antibody or antigen (X)
c. Antibody is a latex particle
d. Antigen is not used at all

A

Latex particle is carrier of antibody or antigen

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3
Q

Positive reaction in tube agglutination test is:

a. Any presence of sediment at the bottom
b. The white clumping on the bottom (with shape of umbrella) and the clear
supernatant (X)
c. The white sediment on the bottom and the turbid supernatant
d. Only the turbidity is formed in the solution

A

The white clumping on the bottom (with shape of umbrella) and the clear supernatant

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4
Q

The precipitation band has the colour

A

White

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5
Q

Zone of equivalence is:

a. Place, where concentration of antigen is in balance with concentration of antibodies
(X)
b. Place, where concentration of antigen is not in balance with concentration of antibodies
c. Place where is abundance of antigens
d. Place where is abundance of antibodies

A

Place, where concentration of antigen is in balance with concentration of antibodies

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6
Q

Draw the scheme of precipitation line for
(NOTES)

A

Antiserum has been carefully laid over an antigen solution for example in tube. At the point of contact, a cloudy or oaque zone forms = precipitation line

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7
Q
  1. Draw the scheme of sandwich ELISA for detection of antigen
    (NOTESS)
A

notesss

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8
Q

Which of the following components is for ELISA?

a. Antibody labelled with fluorescein
b. Antibody labelled with pyocianin
c. Antibody labelled with enzyme (X)
d. Antibody labelled with radioisotope

A

Antibody labelled with enzyme

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9
Q

What is the ratio – blood: H2O : 2.7 % NaCl used for lysis of erythrocytes in procedure of separation of all leucocytes from whole blood?

A

1:2:1

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10
Q

Leucocytes are counted:

a. In 50 medium squares of Burker chamber (X)
b. In all burker chamber
c. Only in one big square of Burker chamber
d. In 20 small squares of Burker chamber

A

In 50 medium squares of Burker chamber

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