Deck 12

Question 1

For evaluation of differential white cell count, the blood smear is stained by:

a. Gram
b. Turk
c. Pappenheim (X)
d. Ziehl-Nielsen

Answer 1

Pappenheim

Question 2

Which immune cells are professional phagocytes?

a. Mono, lympho, baso
b. Neutro, mono/macro (X)
c. Macro, baso, NK, eosino
d. All mononuclear cells

Answer 2

Neutro, mono/macro

Question 3

Granules in eosinophils stained by Pappenheim are

Answer 3

Red

Question 4

In test for respiratory burst activity in phagocytes tetrazolium salt is:

a. Reduced into colorless formalin
b. Oxidized into colored NTB
c. Reduced into formamide
d. Reduced into a coloured formazan (X)

Answer 4

Reduced into a coloured formazan

Question 5

Immune responses of organisms can be detected in two general ways. They are?

Answer 5

a) Detection of an antigen by the help of specific antibody

b) Detection of the presence of specific antibodies in serum → determination whether
animal has been previously exposed to a specific antigen

Question 6

Define antigens (Ag) and antibodies (Ab)

Answer 6

• Antigens (Ag): molecules that stimulate immune response
• Antibodies (Ab): antigen-binding proteins secreted by plasma cells or present on the B-cell membrane
• extra info: injection of Ag into a body → production of Ab → they form a complex and Ag destruction by help of other immune mechanisms occur

Question 7

The three tests that are most applied in agglutination include

Answer 7

1. Slide agglutination: direct and indirect or passive (latex agglutination)
2. Tube agglutination
   3.Haemagglutination

Question 8

Positive result in slide agglutination is identified by

Answer 8

Visible clumping with clearing of the suspension

Question 9

Tube agglutination is a direct agglutination and can be readily used to

Answer 9

Determine titer of antibodies

Question 10

Negative agglutination

Answer 10

Solid “button” sediment with turbid supernatant