Deck 7

Question 1

Draw the scheme for direct IFA
(in notes as can’t draw here)

Answer 1

notes

Question 2

The indirect IFA is most commonly used for

Answer 2

Testing of autoimmune disorders

Question 3

Flow cytometry is used for

Answer 3

Analysis of the cells based on their differential ability to bind fluorescence labelled antibody

Question 4

Which of the following immunoblotting assays is for detection of protein?

Answer 4

Western blotting

Question 5

The essential steps of Western blotting are

Answer 5

BSE Electrophoresis, transfer and immunostaining

Question 6

The essential step for Western blotting diagnosis of BSE is

Answer 6

Treatment of the sample with proteinase K

Question 7

Sensitized erythrocytes are

Answer 7

Erythrocytes labelled antibody (amboceptor)

Question 8

For testing of complement activation is NOT used

Answer 8

Enzyme labelled antibodies specific to complement

Question 9

Complement fixation test is used for

Answer 9

Only for determination of antigen or antibody presence

Question 10

In complement fixation test is positive result

Answer 10

Inhibition of haemolysis of sensitised erythrocytes