Deck 7

Question 1

Draw the scheme for direct IFA
(in notes as can’t draw here)

Answer 1

notes

Question 2

The indirect IFA is most commonly used for:

a. Testing of autoimmune disorders
b. Determination of viral antigen in tissue
c. Determination of bacterial and viral antigen in tissue
d. Determination of CD antigen

Answer 2

Testing of autoimmune disorders

Question 3

Flow cytometry is used for:

a. Analysis and sort a mixture of the cells based on their differential ability enzymelabelled
antibody
b. Analysis of the cells based on their differential ability to bind fluorescence labelled
antibody
c. Reading of absorbance
d. Determination of CD antigen on the cell surface

Answer 3

Analysis of the cells based on their differential ability to bind fluorescence labelled
antibody

(Determination of CD antigen on the cell surface - correct also but B is bertter)

Question 4

Which of the following immunoblotting assays is for detection of protein?

a. Western blotting
b. Southern blotting
c. Eastern blotting

Answer 4

Western blotting

Question 5

The essential steps of Western blotting are:

a. Transfer, electrophoresis and immunostaining
b. BSEElectrophoresis, transfer and immunostaining
c. Electrophoresis, immunostaining and transfer
d. Immunostaining, electrophoresis and transfer

Answer 5

BSE Electrophoresis, transfer and immunostaining

Question 6

The essential step for Western blotting diagnosis of BSE is:

a. Electrophoresis of sample
b. Treatment of the sample with proteinase K
c. Transfer of sample to nitrocellulose filter (NF)
d. Treatment of NF with proteinase K

Answer 6

Treatment of the sample with proteinase K

Question 7

Sensitized erythrocytes are:

a. Erythrocytes labelled by complement
b. Erythrocytes labelled antibody (amboceptor)
c. Erythrocytes labelled enzymes
d. Erythrocytes labelled by fluorescein

Answer 7

Erythrocytes labelled antibody (amboceptor)

Question 8

For testing of complement activation is NOT used:

a. Amboceptor
b. Patient’s serum
c. Enzyme labelled antibodies specific to complement
d. Sensitised erythrocytes

Answer 8

Enzyme labelled antibodies specific to complement

Question 9

Complement fixation test is used for:

a. Only for determination of antigen or antibody presence
b. Only for determination of antibody concentration
c. Only for determination of antigen concentration

Answer 9

Only for determination of antigen or antibody presence

Question 10

In complement fixation test is positive result:

a. Haemolysis of sensitised erythrocytes
b. Inhibition of haemolysis of sensitised erythrocytes
c. Lysis of complement
d. Inhibition of lysis of complement

Answer 10

Inhibition of haemolysis of sensitised erythrocytes