Deck 8

Question 1

Number leukocytes we may determinate by counting of cells by

Answer 1

Microscope and Burker chamber

Question 2

The blood in which we want determinate the number of leukocytes is

Answer 2

Diluted in Turk solution (1:20)

Question 3

We count the leukocytes

Answer 3

In 50 medium squares of Burker chamber

Question 4

For checking of viability of cells we may use

Answer 4

Trypan blue solution
(tryptan?)

Question 5

The live cells are cells

Answer 5

Never stained

Question 6

Mononuclear cells are

Answer 6

Monocytes, macrophages and lymphocytes

Question 7

The common characteristic of separating solution for isolation of mononuclear cells is

Answer 7

Density (1.075-1.0077 g/ml)

Question 8

If we use separating solution for isolation of mononuclear cells, we need

Answer 8

Gently overlay separating solution with blood and then centrifuge the samples (2500 rpm/15 min)

Question 9

Phagocytosis is property of

Answer 9

Neutrophils and monocytes/macrophages

Question 10

Which of the following statements is FALSE:

a. Phagocytosis is complex process
b. Phagocytes arrive at a site of inflammation of chemotaxis
c. Each phagocytic cells is antigen presenting cell (APC)
d. Lysosome fuse with phagosome to form a phago-lysosome

Answer 10

says D on past paper but am sure none are false…?