Deck 8

Question 1

Number leukocytes we may determinate by counting of cells by:

a. Microscope
b. Microscope and Burker chamber
c. Burker chamber
d. Spectrophotometer

Answer 1

Microscope and Burker chamber

Question 2

The blood in which we want determinate the number of leukocytes is:

a. Diluted in PBS (1:1)
b. Diluted in distilled water (1:1)
c. Diluted in Turk solution (1:20)
d. Non diluted

Answer 2

Diluted in Turk solution (1:20)

Question 3

We count the leukocytes:

a. In 50 medium squares of Burker chamber
b. In all Burker chamber
c. Only in one big square of Burker chamber
d. In 3 big squares of Burker chamber

Answer 3

In 50 medium squares of Burker chamber

Question 4

For checking of viability of cells we may use:

a. Turk solution
b. Trypan blue solution
c. Tryptophan solution
d. Toluidin blue solution

Answer 4

Trypan blue solution
(tryptan?)

Question 5

The live cells are cells:

a. Distained
b. Sometimes stained
c. Never stained
d. Usually stained

Answer 5

Never stained

Question 6

Mononuclear cells are:

a. Monocytes, macrophages and lymphocytes
b. Only monocytes and macrophages
c. Only lymphocytes
d. All WBCs

Answer 6

Monocytes, macrophages and lymphocytes

Question 7

The common characteristic of separating solution for isolation of mononuclear cells is:

a. Density (1.075-1.0077 g/ml)
b. Opacity (1.075-1.0077 g/ml)
c. Turbidity (1.075-1.0077 g/ml)
d. Absorbance (1.075-1.0077 g/ml)

Answer 7

Density (1.075-1.0077 g/ml)

Question 8

If we use separating solution for isolation of mononuclear cells, we need:

a. Mix blood with separating solution, then incubate samples for 15 minutes @ room
temperature
b. Vortex blood with separating solution and then centrifuge the samples (2500
rpm/15min)
c. Gently overlay separating solution with blood and then centrifuge the samples (2500
rpm/15 min)
d. Lysed blood with distilled water and then centrifuge the samples (2500 rpm/15 min)

Answer 8

Gently overlay separating solution with blood and then centrifuge the samples (2500 rpm/15 min)

Question 9

Phagocytosis is property of:

a. All WBC’s
b. All mononuclear cells
c. Only neutrophils
d. Neutrophils and monocytes/macrophages

Answer 9

Neutrophils and monocytes/macrophages

Question 10

Which of the following statements is FALSE:

a. Phagocytosis is complex process
b. Phagocytes arrive at a site of inflammation of chemotaxis
c. Each phagocytic cells is antigen presenting cell (APC)
d. Lysosome fuse with phagosome to form a phago-lysosome

Answer 10

Each phagocytic cells is antigen presenting cell (APC)

Question 11

In blood of different animals we usually have –

a. At least 6-10x105 leukocytes
b. 6-10x105 leukocytes
c. Approx. 6-10x106 leukocytes
d. At least 6-10x107 leukocytes

Answer 11

c. Approx. 6-10x106 leukocytes