Creatine kinase Flashcards
Why do cells die when there is a lack of oxygen?
Oxygen is needed to produce enough ATP to maintain ion pumps by supplying ATPases with ATP. As soon as the ion balance is lost cells die.
Which cells have the most creatine kinase?
Metabolically active tissues.
Creatine kinase structure?
Creatine kinase is a dimer. - Made up of two monomers.
How many isoforms of each monomer in creatine kinase are there? What are the isoforms called?
2 - B and M.
How many creatine kinase isoenzymes are there?
3
What creatine kinase form can be found in the brain?
BB.
What creatine kinase form can be found in the muscles?
MM.
What creatine kinase form can be found in the cardiac muscle fibres?
BM.
What serum markers can be used for a cardiac infarction?
Serum glutamate oxaloacetate transaminase (SGOT)
lactate dehydrogenase (LDH)
Cardiac troponin T
Creatine kinase.
How can creatine kinase activity in serum be detected?
A coupled assay. Absorption spectra of NADP+ and NADPH
What is the best diagnostic marker for cardiac infarction 5 days after infarction?
LDH
What is the best diagnostic marker for cardiac infarction 1-2 days after infarction?
Creatine kinase.
What are the most specific markers for cardiac infarction?
Cardiac troponin I and troponin T.
