Chymosin-mediated coagulation of milk.

Question 1

What is chymosin?

Answer 1

a proteolytic enzyme synthesized by the chief cells in the stomach.

Question 2

Another name for chymosin is?

Answer 2

Rennin

Question 3

Role of chymosin in digestion?

Answer 3

to coagulate milk in the stomach, important in juvenile animals.

Question 4

What will happen if milk is not coagulated?

Answer 4

Flows through rapidly and can not be digested by stomach enzymes.

Question 5

When is chymosin secretion at it’s highest?

Answer 5

during the first few days after birth, then declines and is replaced by secretion of pepsin.

Question 6

What is the major gastric protease?

Answer 6

Pepsin

Question 7

How does chymosin work?

Answer 7

it proteolyse and inactivates kappa casein, converting it to para-kappa- casein –> smaller protein called macropeptide.
Para- kappa-casein can not stabilize micelles and the calcium-insoluble caseins precipitate –> curd.

Question 8

Chymosin -Casein formula?

Answer 8

Chymosin + casein = soluble paracasein

Paracasein + Ca2+ = insoluble paracasein calcium.

Question 9

What is the function of sodium oxalate?

Answer 9

It works as an anticoagulant (like EDTA in blood)

Question 10

What will happen when adding Ca2+ to milk that is balanced (treated with anticoag.)?

Answer 10

Ca2+ wil work as an coagulant and restore coagulation –> debalance

Question 11

What will give best result.. natural or mediated coagulation?

Answer 11

Natural conditions