Chromatographie (Biochimie) Flashcards
Chromatography assessment of glycated hemoglobin most likely used which type of chromatography:
- ion exchange
- affinity
- partition
- adsorption
2 - affinity
The type of chromatography that involves use of immunological principles is:
- ion exchange
- affinity
- partition
- adsorption
2 - affinity
In the thin layer chromatography procedure for drug screening, the retention factor value for a compound is given by the:
- ratio of distance moved by the unknown solute to distance moved by the solvent in the mobile phase
- rate of movement of the mobile phase through the adsorbant compared with standards and controls
- measurement in centimeters of the distance the solute moved in the mobile phase of from the point of application
- distance moved by the mobile phase front from the point of application compared with a control
1 - ratio of distance moved by the unknown solute to distance moved by the solvent in the mobile phase
A highly sensitive and specific chromatographic method that provides quantitative results concerning serum drug concentration and uses a fixed-loop injector to send the mobile phase into the column is:
- TLC
- gas chromatography
- high performance liquid chromatography
- mass spectrometry
3 - high performance liquid chromatography
A toxicology screen is set up using thin layer chromatograhic plates. Four positive controls/standards are used with the following distance of migration from application point results: Std A = 8cm; Std B = 15 cm; Std C = 22 cm. The acetone solvent front moved 35 cm. After processing an unkown sample, a solute had a migration distance of 16 cm. What are the retention factors of Standards A, B and C.
A=0.23
B=0.43
C=1.59
A toxicology screen is set up using thin layer chromatographic plates. Four positive controls/standards are used with the following distance of migration from application point results: A = 8cm; B=15cm; C=22cm. The acetone solvant from moved 35cm. After processing an unkown sample, a solute had a migration distance of 16cm. What is the Rf of the unkown solute.
0.45
A toxicology screen is set up using thin layer chromatographic plates. Four positive controls/standards are used with the following distance of migration from application point results: A = 8cm; B=15cm; C=22cm. The acetone solvant from moved 35cm. After processing an unkown sample, a solute had a migration distance of 16cm. Which one of the standards has a similar Rf to that of the unknown solute:
Standard B
In a gas liquid partition chromatography, what constitutes the components of the stationary phase:
- the inert gas is the stationary phase
- packed columns containing uncoated support particles
- methyl silicone polymers coated on capillary tubes
- stainless steel columns packed with graphitized carbon
3 - methyl silicone polymers coated on capillary tubes
In HLPC, analyte detectors can consist of fluorometers, photometers, and electrochemical detectors. One example of a measurement of electrochemical detection is performed by:
- monitoring the current that is generated by oxidation or reduction reaction under a constant potential voltage and is proportional to the concentration of analyte
- measurement of an electrical potential difference between two electrodes
- measuring the radiant energy iven off when the chemical bonds of an element are dissociated and the element is placed in a ground or atomic state
- measurement of both an alteration in enzyme level and a redox reaction at a potentiometric ion specific electrode
1 - monitoring the current that is generated by oxidation or reduction reaction under a constant potential voltage and is proportional to the concentration of analyte
You are preparing placental tissue for separation and isolation of a specific enzyme. The enzyme has a negative charge, so you want to use chromatography to perform your task. Following homogenization, the tissue is reduced to a large volume of liquid. Which one of the basic types of chromatography would be most useful for your situation:
- planar
- column
- gas
- reverse
2 - column
You are preparing placental tissue for separation and isolation of a specific enzyme. The enzyme has a negative charge, so you want to use chromatography to perform your task. Following homogenization, the tissue is reduced to a large volume of liquid. Which one of the following separation mechanisms would be most useful:
- ion exchange
- partition
- affinity
- adsorption
1 - ion exchange
You are preparing placental tissue for separation and isolation of a specific enzyme. The enzyme has a negative charge, so you want to use chromatography to perform your task. Following homogenization, the tissue is reduced to a large volume of l8iquid. Because of budget cuts, you cannot purchase the correct packing material for your experiment. You have found, however, some cross-linked dextran in the laboratory that might work based on a different principle of separation. Which one of the following separation techniques could be used instead if you were looking to separate the molecule based on molecular weight:
- partition
- affinity
- adsorption
- size exclusion
4 - size exclusion
