Chapter 6 (pdf 17) Flashcards
uses reducing power to generate ATP
respiration
NADH and FADH2 transfer electrons to produce proton motive force
respiration
allows for recycling of electron carriers*
respiration
electrons reduce terminal electron acceptor
respiration
oxygen is in aerobic respiration
respiration
aerobic respiration uses another organic molecule
respiration
itself generates no ATP only ATP is from glycolysis/TCA
fermentation
does allow for recycling of NADH
fermentation
oxygen and streptococcus both like
fermentation
partial oxidation of glucose
fermentation
recycles NADH pool
fermentation
uses pyruvate or derivative as terminal electron acceptor
fermentation
used by organisms that can not respire
fermentation
organisms can not respire during fermentation due to
lack of suitable inorganic electron acceptor or lack of transport chain
only ATP produced is by glycolysis or TCA
fermentation
other steps for consuming excess reducing power in fermentation
- recycles NADH
- fermentation pathways use pyruvate or derivative as terminal electron acceptor
end products of fermentation
Lactic acid – Ethanol – Butyric acid – Propionicacid – 2,3-Butanediol – Mixed acids --all are produced in a series of reaction to produce appropriate terminal electron acceptors
pyruvate acts as the terminal electron acceptor
lactic acid
pyruvate converted o lactate by LDH
lactic acid
produces lactic acid
e.g Streptococcus, Lactococcus or Enterococcus
Homolactic fermentation
produced lactic acid, alcohol, and CO2
• e.g Leuconostoc, Oenococcus, Weissella
Heterolactic fermentation
pyruvate loses CO2
Ethanol
Acetylaldehyde to Alcohol
•NADH to NAD+
Ethanol
Beer, wine, spirits and biofuel
• Saccharomyces
• Zymonmonas
Ethanol
Produced by obligate anaerobes
e.g. Clostridium sp
Butyric acid
Converted to ‘fuel’ in form of butanol and acetone
Butyric acid
•e.g Propionibacterium used for Swiss
cheese
• Propionic acid formation
•e.g. Aspergillus used flavoring in soda
• Citric acid
two pyruvate convert to acetonin
2,3 Butanediol
differentiate Enterobactericeae
e.g. Klebisella vs E. coli
2,3 Butanediol
Voges-Proskauer test
2,3 Butanediol
Acetic acid, lactic acid, succinic acid
•Mixed acids
Methyl-red test
• E. coli vs Enterobacter
•Mixed acids