Chapter 6 Flashcards
Fluid mosaic model
The general structure of membranes is known as the fluid mosaic model
Cholesterol
-is a flat hydrophobic molecule
-Cholesterol in eukaryotic cell membranes prevents “close packing” of phospholipids
-at colder temperatures membrane still maintains fluid-like motion
-conversely, at high temperatures the hydrophobic cholesterol helps hold membrane together
-temperature adaptation in some animals involves adjusting the amount of cholesterol in their membranes
Fluidity depends on
-temperature, lipid composition (saturated vs unsaturated), ad cholesterol percentage
Peripheral membrane proteins
Lack exposed hydrophobic groups and do not penetrate the bilayer. They may be anchored to lipid groups however
Integral membrane protiens
have hydrophobic and hydrophilic regions or domains. Some extend across the lipid bilayer (transmembrane); others are partially embedded
Transmembrane proteins
extend all the way through the phospholipid bilayer
domain
different parts of the protein that have different functions
anchored membrane proteins
membrane proteins that have fatty acids or other lipid groups covalently attached
Lipid-rafts
While some membrane proteins can move freely within the bilayer, others are anchored to a specific region
-Free diffusion is limited by the cytoskeleton and protein-protein interactions
Freeze-fracturing
a technique that reveals proteins that are embedded in the phospholipid bilayers of cellular membranes. Electron microscopy is then used to examine the structure
fluorescent microscopy
can be used to visualize “tagged” membrane proteins and lipids
Patch-Clamp
experimentation creates membrane “plugs”
Functions of membrane proteins
-Process information (signals and signaling receptors)
-Organize chemical reactions
-Transform energy
-Cell recognition and adhesion
-Transport into and out of the cell
Information processing
Binding of a specific ligand can initiate, stop, or modify cell functions.
Organizing chemical reactions
-Many cellular processes involve a series of enzyme-catalyzed reactions- all the molecules must come together for these to occur. Forms an “assembly line” of enzymes
Some transform energy…
Inner mitochondrial membranes-energy from fuel molecules is transformed to ATP
-Thylakoid membranes of chloroplasts transform light energy to chemical bonds
Cell recognition and adhesion
-Membranes also have carbohydrates on the outer surface that serve as recognition sites for other cells and molecules
-Glycolipids- carbohydrate+lipid
-Glycoproteins-carbohydrate+protein
Homotypic
The same molecule sticks out from both cells and forms a bond
Heterotypic
the cells have different protiens
Cell junctions
specialized structures that hold cells together
Tight junctions
help ensure directional movement of materials
-a quilted seal, air and water tight
Desmosomes
link adjacent cells tightly but permit materials to move around them in the intercellular space, similar to “spot welds”
Gap junctions
let adjacent cells communicate