Chapter 3 - Biochemical Processes in Cells Flashcards
cellular metabolism
the sum total of chemical reactions that occur in each living cell
anabolic reactions / endergonic
chemical reaction in which atoms and molecules are joined together to make more complex molecules
energy required
e.g. photosynthesis
catabolic reactions / exergonic
reactions that break down complex molecules into simpler molecules
release energy
e.g. cellular respiration, digestion
ATP
adenosine triphosphate
contains adenosine and 3 phosphate
renewable energy source
ADP
adenosine diphosphate
contains adenosine and 2 phosphate
phosphorylation
the addition of a phosphate group to an organic molecule
ADP + Pi = ATP
enzyme
proteins which are capable of catalysing (speeding up) biochemical reactions by reducing the activation energy
biological catalysts
specific: catalyse only one type of reaction
recyclable: remains unchanged, used again
active site
the region of the enzyme to which the substrate binds
substrate
compound on which an enzyme acts
enzyme substrate complex
combination of the enzyme and its substrate
lock and key model
substrate fits into the active site perfectly
rigid active site
highly specific
Induced fit model
active site changes shape slightly to fit the substrate
flexible
increases range of substrate specificity
activation energy
the amount of energy needed to start a chemical reaction
enzymes lower activation energy
factors affecting enzyme activity
pH
temperature
enzyme and substrate concentrations
inhibition
factors affecting enzyme activity
pH
enzyme denatured in extreme pH
buffered solutions
factors affecting enzyme activity
temperature
enzymes work best in temp they’re found in
temp increases: molecules more excited and collide more often
denaturing
temp too low: little to no activity
denaturing
hydrogen bond broken by heat
protein loses 3D shape: active site changed
irreversible
factors affecting enzyme activity
enzyme and substrate concentrations
increases substrate: more product until enzyme worked to full capacity
increased enzyme: increases yield of the production until substrate all used up
factors affecting enzyme activity
inhibition
chemical substances that interfere with enzyme function
inhibitors
compete with the substrate for the active site
competitive and non-competitive
competitive inhibitors
can fill the active site of the enzyme preventing it from binding with the substrate
non-competitive inhibitors
can attach to the enzyme causing a change in the shape of the active site