Chapter 11 - Gene Function: Genes in Action Flashcards
protein synthesis
process by which individual amino acids are connected to each other in a specific order dictated by the nucleotide sequence in DNA
transcription and translation
transcription
process of copying the genetic instructions present in DNA to mRNA
steps of transcription
RNA polymerase unwinds and exposes bases of DNA template strand
RNA polymerase moves along template strand
Complementary nucleotides brought into place -> pre-mRNA
Introns removed -> mRNA
RNA processing
pre-mRNA is altered after transcribed methylated cap added poly-A tail added introns removed final mRNA transcript leaves nucleus to cytoplasm
translation
the building of a polypeptide chain from amino acids guided by the sequence of codons contained in the mRNA
steps of translation
Ribosomes read mRNA for start codon (TAC)
Ribosome moves along mRNA
tRNA anticodons attach to complementary mRNA codons
Amino acids move into position and peptide bond with adjacent amino acid
Stop codon
Protein/polypeptide produced
gene regulation
cells don’t express all genes at same time
genes can be switched on and off
save energy
gene switching on and off
DNA binding proteins bind ot regions of nuclear membrane and directly switch genes on or off
signalling proteins bind to receptors on membranes of cells and trigger intracellular responses to which on or off
gene expression is dependent of what
type of cell
stage of development
conditions within and around the cell
DNA self replication
precedes cell division
unwinds and free nucleotides pair with complementary bases