Chapter 18: Gene Expression Flashcards
Operon consists of…
a promoter, operater, and structural genes
Promoter
where RNA polymerase attaches signalling the start of the gene
Operator
where a repressor binds, stopping the transcription of that gene
Structural Genes
genes coding for the enzyme, transcribed as a unit
TRYP Operon in E.Coli
exsists in the “ON” state, controls production of tryptophan
LAC Operon in E.Coli
exsists in the “OFF” state, turns on when lactose is present
Repressible Operon
is normally ON but can be turned off (TRYP OPERON)
Inducible Operon
is normally OFF but can be turned on (LAC OPERON)
Recombinant DNA Tech
DNA from different sources is combined (human DNA + bacterial DNA)
Polymerase Chain Reaction (PCR)
creates millions of copies of a gene (or segment of DNA) using DNA polymerase
Gene Amplification
DNA must be heated to seperate strands