Chapter 18: Gene Expression Flashcards

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1
Q

Operon consists of…

A

a promoter, operater, and structural genes

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2
Q

Promoter

A

where RNA polymerase attaches signalling the start of the gene

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3
Q

Operator

A

where a repressor binds, stopping the transcription of that gene

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4
Q

Structural Genes

A

genes coding for the enzyme, transcribed as a unit

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5
Q

TRYP Operon in E.Coli

A

exsists in the “ON” state, controls production of tryptophan

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6
Q

LAC Operon in E.Coli

A

exsists in the “OFF” state, turns on when lactose is present

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7
Q

Repressible Operon

A

is normally ON but can be turned off (TRYP OPERON)

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8
Q

Inducible Operon

A

is normally OFF but can be turned on (LAC OPERON)

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9
Q

Recombinant DNA Tech

A

DNA from different sources is combined (human DNA + bacterial DNA)

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10
Q

Polymerase Chain Reaction (PCR)

A

creates millions of copies of a gene (or segment of DNA) using DNA polymerase

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11
Q

Gene Amplification

A

DNA must be heated to seperate strands

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