Chapter 11: Sterilization Flashcards

1
Q

What is the Spaulding classification regarding instruments for sterilization?

A
  • Critical - come into contact with the bloodstream or sterile tissue (instruments, needles, catheters should be sterile)
  • Semi-critical - Come into contact with mucous membranes or non-intact skin (ETT, laryngoscopes, high-level disinfection)
  • Non-critical - Contact only intact skin (stethoscope, BP cuff, intermediate level disinfectants)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is toxic anterior segment syndrome (TASS)?

A

Uveitis from not washing all the cleansers off ophtho instruments.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the main options for cleaning of surgical instruments prior to sterilization?

A
  • Manual cleaning with enzymatic cleaners or detergents (no bubbles)
  • Mechanical cleaning of surgical instruments (preferred method)
    Ultasonic cleaning with cavitation, washer decontaminators, washer disinfectors and washer sterilisers. Instruments must be thoroughly rinsed after cycle is complete
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the only approved method for drying instruments prior to sterilization?

A

Filtered, medical grade compressed air

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What are the (9) ideal properties of a wrapping material?

A
  • Prevent microbial penetration
  • Permeable to the sterilising agent
  • Resistant to heat
  • Allow for aeration
  • Resistant to physical damage and moisture penetration
  • Possess minimal wrap memory
  • Long shelf-life with sufficient strength and durability
  • Free of toxic ingredients and dyes
  • Provide a barrier to microorganisms and fluid

Does not exist!

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the most common wrap material? What are its downsides?

A

Cotton muslin wrap
- Can generate lint
- Not moisture resistant
- Requires a double layer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

List the methods available for sterilization:

A
  • Liquid chemical germicides (cold sterilization)
  • Steam sterilization
  • Ethylene oxide sterilization
  • Ozone sterilization
  • Plasma sterilization
  • Vaporized Hydrogen Peroxide
  • Sterilization by radiation
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

List some options for cold sterilization. What are the problems with cold sterilization?

A
  • Glutaraldehyde 1-1.5%
  • Orthophthalaldehyde (Cidex)
  • Hydrogen peroxide 7.5%

Should be considered high level disinfectants rather than sterilization. Glutaraldehyde is irritating to skin and can decrease pulmonary function.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How do steam sterilizers work?

A

Kill microorganisms through coagulation and denaturation of proteins by moist heat
- Water serves as a catalyst and allows lower temperatures to be used

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What are the 2 types of steam sterilizers?

A
  • Gravity-displacement (steam rises to the top and cool air is removed though a valve at the bottom)
  • Prevacuum type - A pump is used to remove air before steam is admitted to allow for very rapid and uniform steam distribution
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are the temperature and time options for gravity-displacement autoclaves?

A
  • 121C for 30 minutes with 15-30mins drying
  • 132C for 15 minutes with 15-30mins drying
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What are the potential causes of wet packs after steam sterilisation?

A
  • Improper loading
  • too large metal mass
  • Overloaded tray
  • Lack of absorbent materials
  • Insufficient cooling time
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the recommended temp and time for dry heat sterilisation? When is this indicated?

A
  • 160C for 120 mins
  • Certain sharp instruments, glassware and powders that can be damaged by moisture
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are the guidelines for flash sterilisation of a metallic, unwrapped, nonporous item in a gravity-displacement autoclave?

A

135C for 3min with 1 min drying
“immediate use steam sterilization” is for emergencies, burns can result from temperature. Not for implantable devices.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are the safety concerns surrounding ethylene oxide?

A

Carcinogenic, mutagenic and neurologic hazards

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is ozone sterilization used for? What is the recommended temp and time?

A
  • Good option for moisture and heat-sensitive items.
    -NO wood/paper
  • 30-35C for 4 hours
17
Q

What is plasma sterilization?

A
  • Use of electromagnetic energy to create a plasma phase from a vapor of hydrogen peroxide, oxygen or peracetic acid/H2O2mix
  • Rapid and cost effective
  • 45-59C for 28-75min
    NO linens, liquids, wood
18
Q

What is used for radiation sterilization?

A

Cobalt 60 gamma rays or electron accelerators
Suture and implants
Expensive

19
Q

What are the 6 classes of sterilization indicators?

A
  • Class 1 - Indicates the pack has been processed but limited info regarding parameters met - eg external tape
  • Class 2 - Test for air removal (“Bowie-Dick test”)
  • Class 3 - React to a specific parameter
  • Class 4 - React to more than 1 parameter
  • Class 5 - React to all parameters which are important for a certain type of sterilization
  • Class 6 - Monitor more specific guidelines for unique parameters (eg combatting prion infection)

Recommended to always use a class 1 indicator as well as an additional indicator in the deepest part of the pack

20
Q

What biological indicator is used for steam sterilisation (as well as plasma and ozone)

A
  • Geobacillus Stearothermophilus
  • One study found 12% failure rate on biological indicators, none of which were detected by simultaneous chemical indicators
21
Q

What are the ideal conditions for storage of sterilized materials?

A
  • Temp should not exceed 24C
  • Humidity should not exceed 70%
  • 4 air exchanges per hour
  • Ideally in closed cabinets
    As long as the sterile wrap remains sealed, undamaged and dry, internal sterility should be maintained indefinitely
22
Q

What should you label your sterilized pack with?

A

Instrument types
Sterilization date
Expected expiration date
Name/initials of staff who sterilized

23
Q

After autoclaving, small instruments in double wrapped linen or double pouched are sterile for how long with appropriate storage?

A

96 weeks (24 months / 2 years)

24
Q

After EtOx sterilization, how long are heat-sealed plastic wrapped items sterile for?

A

12 months