Chap. 27 Book Flashcards
What were the first organisms to inhabit earth?
Prokaryotes
Are most prokaryotes unicellular or multicellular?
Unicellular
What is the difference between the cell wall of prokaryotes and eukaryotes?
Prokaryotes have a cell wall which contains peptidoglycan
A polymer composed of modified sugars cross-linked by short peptides. Why is this term important to know?
Peptidoglycan
…encloses the entire prokaryotic bacterium
Name the three most common shapes of prokaryotes
Cocci, bacilli, Spirilli
What is the purpose of the Gram stain?
To categorize bacterial species according to differences in wall composition
What type of cell wall do gram-positive bacteria have?
Simple walls
Large amounts of peptidoglycan
A bacteria is structurally complex and has an outer membrane that contains lipolysaccharides. A gram stain is done. What will the result be?
Gram-negative
Why are gram-negative bacteria more resistant than gram-positive species to bacteria?
outer membrane impedes entry of drugs
A dense and well-defined and sticky layer of polysaccharide or protein
capsule
The Streptococcus bacterium attaches to cells in the respiratory tract. How does it do this?
capsule
What advantages does a capsule give to a prokaryote?
- adherence to substrate or individuals in a colony
- protect against dehydration
- shield from attacks by host
Certain bacteria develop resistant cells called _____ when they lack an essential nutrient
endospores
ENDOSPORE FORMATION
a. original bacteria cell produces a copy of its ___ and surrounds that copy with a tough multilayered structure = __________.
chromosome, endospore
What is the difference between fimbriae and pili? What is their common function?
Fimbriae are shorter and more numerous, connect two cells together
Fimbriae
a. hairlike appendages that attach cells to surfaces or e/o
b. appendages that pull two cells together prior to DNA transfer
a. hairlike appendages that attach cells to surfaces or e/o
Of the various structure that enable prokaryotes to move, the most common are what?
FLagella
What do structural and molecular comparisons indicate about the flagella of bacteria, archaea and eukaryotes? Would they be analogous or homologous?
they arose independently, analogous
T/F all prokaryotes are capable of taxis
False (50% are)
How could such a complex structure like the flagellum evolve?
bacterial flagellum originated as simpler structures that were modified in a. stepwise fashion over time
The bacterial flagellum may have evolved as other proteins were added to an ancestral secretory system. What is this process an example of?
Exaptation
The process in which existing structures take on new functions through descent with modification
Exaptation
The cells of prokaryotes are ______ than those of eukaryotes in their internal structure and DNA arrangement
simpler
Unlike eukaryotes, what do prokaryotes lack?
nucleus
a region of cytoplasm that is not enclosed by a membrane
nucleoid
smaller rings of independently replicating DNA molecules
plasmids
What two places carry genes in a prokaryote?
nucleoid, plasmids
What is the process of reproduction for a prokaryote?
binary fission
3 Key Features of Prokaryote Biology
a. they are ____
b. they reproduce by __________
c. they have ______ generation times
small, binary fission, short
Concept Check: rapid _______, _______ and _______ promote genetic diversity in prokaryotes
reproduction, mutation, genetic recombination
What is evidence that prokaryotes are not “primitive” or “inferior” in an evolutionary sense?
they rapidly adapt to new conditions
the combining of DNA from two sources
genetic recombination
How does genetic recombination occur in prokaryotes?
transformation, transduction, conjugation
What brings together prokaryotic DNA from different cells? If the cells are of different species, what is the specific term for this?
genetic recombination (transformation, transduction, conjugation), horizontal gene transfer
The genotype and even phenotype of a prokaryotic cell is altered by the uptake of foreign DNA from its surroundings
Transformation
A harmless strain of Streptococcus pneumonia can be transformed into pneumonia-causing cells if the cells are exposed to DNA from a pathogenic strain. What type of genetic recombination is here?
Transformation
Phages can carry prokaryotic genes from one host cell to another.
Transduction
An E. coli donor cell extends a pilus that attaches to a recipient cell.
Conjugation
DNA is transferred between two prokaryotic cells that are temporarily joined.
Conjugation
Why is the F factor important for conjugation-genetic recombination?
It consists of 25 genes
F plasmid-cells function as DNA donors
F- cells are DNA recipients
Chromosomal genes can be transferred during conjucation when the donor cell’s F factor is integrated into the chromosome
plasmids which carry resistance genes that code for enzymes that specifically destroy or hinder the effectiveness of antibiotics
R plasmids
CC 27.2
“Although rare on a per gene basis, new mutations can add considerable genetic variation to prokaryotic populations in each generation. Explain how this happens.”
“The large number of individuals in prokaryotic populations makes it likely that in each generation there will be many individuals that have new mutations at any particular gene, thereby adding considerable genetic diversity to the population.”
CC 27.2
“Distinguish between the three mechanisms of transferring DNA from one bacterial cell to another.”
a. Transformation - naked, foreign DNA from the environment is taken up by a bacterial cell
b. transduction - phages carry bacterial genes from one bacterial cell to another
c. conjugation - a bacterial cell directly transfers plasmid or chromosomal DNA to another cell via a temporary mating bridge
CC 27.2
“In a rapidly changing environment, which bacterial population would likely be more successful, one that includes individuals capable of conjugation or one that does not?”
Population with individuals capable of conjugation.
Some of its members could form recombinant cells whose new gene combinations might be advantageous in a novel environment
CC 27.2
“If a non-pathogenic bacterium were to acquire resistance to antibiotics, could this strain pose a health risk to people?”
Yes!
- genes for antibiotic resistance could be transferred (transformation, transduction, conjugation) from the nonpathogenic bacterium to a pathogenic bacterium
CC 27..2
“In general, how does DNA transfer among bacteria affect the spread of resistance genes?”
Transformation, transduction and conjugation tend to increase the spread of resistance genes
Which prokaryotes must use O2 for cellular respiration and cannot grow without it?
obligate aerobes