Ch 2 Microscopy Flashcards
What is resolution?
The smallest distance by which two objects can be separated and still be distinguished
What is detection?
The ability to determine the presence of an object
What is magnification?
An increase in the apparent size of an image to resolve smaller separations between objects
What is the formula for resolution?
λ/2NA
What should the λ and NA look like to increase resolution?
λ- is small
NA- is large
How do you calculate the total magnification?
Ocular mag x objective mag
True or False: Wet mounts allow you to see cells in their natural state
True
What is fixation?
When cells are made to adhere to a slide in a fixed position
What is staining?
Cells are given a distinct color
True or False: a simple stain adds a light color to cells
False, it adds a dark color to cells
True or False: differential staining stains all cells
False, only targets a specific cell
How is gram positive different from gram negative
Gram positive has a thick peptidoglycan cell wall and retains the crystal violet stain after gram staining
Acid fast stains stain which species
Mycobacterium with carbolfuchsin
Spore staining targets which species
Bacillus and Clostridium with malachite green
What is negative staining?
Staining the background to make capsules more visible
