Cervical HPV Dysplasia Carcinoma Flashcards
HPV is attributed to how much % of Cervical, Anal, Penis and Vaginal cancers?
Cervical: 100%
Anal: 90%
vaginal, Penile: 40%
two most prevalent strains of HPV?
HPV -16
HPV-18
highes risk of HPV age?
20-24
14-19
in order to infect: HPV must be where?
contact basement membrane
What are the HPV early genes?
E1-7
Replication and nucleic acids
what are HPV late genes?
L1-major capsids
L2 - minor capsids
HPV replicated where in cell? as what form?
nucleus as circular episomes
where is HPV amplified once infected?
amplified in basal keratinocytes
HPV E6 does what?
deactivated p53 - cell won’t apoptose from infection
HPV E7 does what?
blocks RB genes and allow dysplasia and metaplasia
Prolonged HPV does what to host genome?
integrates itself = high grade and invasive carcinoma
what is a cervical condyloma?
cervix becomes thicken papillomatous
what is a flat cervical condyloma?
keratinocytes are enlarges, darker abnormal appearance, haphazard arrangement
appearance of koilocytes
CIN 1?
basal 1/3 cell disorder
CIN 2?
basasl 2/3 cell disorder
CIN 3?
full thickness of epithelium affected
what do you see in cervical squamous cell carcinoma
irregular and infiltrative ‘nests’
cervical adenocarcinoma in situ apperance?
large atypical nuclei, glandular appearance, polymorphic, no polarity
What is low grade squamous intraepithelial lesion? (LSIL)
CIN1
binucleation
koilocytes
What is high grade squamous intraepithelial lesion? (HSIL)
CIN2,3
large nuclei, hyperchromatic
HPV vaccine design against what in the virus?
late phase proteins L1,2
if abnormal smear, then what?
colposcopic assessment
