Cell Structure and Microscopes

Question 1

What level of detail of a cell can you see using a light microscope?

Answer 1

Minimal detail, the rough outline of a cell and maybe the nucleus and the outline of unidentifiable organelles

Question 2

Nile blue stain can stain which cell feature?

Answer 2

The nucleus

Question 3

Are ribosomes found in prokaryotic cells or eukaryotic cells?

Answer 3

both

Question 4

Does a prokaryotic cell contain RER?

Answer 4

No

Question 5

Does a prokaryotic cell contain a golgi apparatus?

Answer 5

No

Question 6

What are prokaryotic cell walls made out of?

Answer 6

Peptidoglycan

Question 7

Do prokaryotic cells have extrachromosomal DNA that is circular?

Answer 7

Yes

Question 8

What size ribosomes do prokaryotic cells have?

Answer 8

70S

Question 9

Is RER and/or SER found in both plants and animals cells?

Answer 9

both are found in both

Question 10

Is a ribosome a membrane-bound organelle?

Answer 10

No

Question 11

Name of feature which is common to bacteria, fungi and protoctista

Answer 11

ribosomes

Question 12

What is the maximum resolution of a laser scanning confocal microscope?

Answer 12

200 nm

Question 13

Is a laser scanning confocal microscope 2D or 3D?

Answer 13

either

Question 14

What is the image colour of a laser scanning confocal microscope?

Answer 14

coloured

Question 15

What is the maximum resolution of a scanning electron microscope?

Answer 15

3 - 10 nm

Question 16

Does a scanning electron microscope produce 3D or 2D images?

Answer 16

3D

Question 17

What is the image colour of a scanning electron microscope?

Answer 17

black and white

Question 18

What is the maximum resolution of a transmission electron microscope?

Answer 18

0.5 nm

Question 19

Does a transmission electron microscope produce 2D or 3D images?

Answer 19

2D

Question 20

What is the image colour of a transmission electron microscope?

Answer 20

black and white

Question 21

Outline how a student could prepare a temporary mount of tissue for a light microscope

Answer 21

1, Obtain thin section of tissue e.g. using ultratome or by maceration
2, Place plant tissue in a drop of water
3, Stain tissue on a slide to make structures visible
4, Add coverslip using mounted needle at 45 degrees to avoid trapping air bubbles

Question 22

Functions of the golgi apparatus

Answer 22

transport, sorting and modification of both proteins and lipids

Question 23

Cells in culture will absorb amino acids from the surrounding culture medium. If radioactively labelled glycine is provided in the culture medium, in which organelle will radioactivity be found first?

Answer 23

ribosomes

Question 24

Intestinal epithelial cells have observable “brush borders” seen on the cells. What are they?

Answer 24

microvilli

Question 25

When mitochondria are extracted from cells, they are usually kept in a 0.25 moldm-3 sucrose solution. What is the purpose of the sucrose solution?

Answer 25

it prevents the mitochondria from changing structure

Question 26

Which one of the following has the lowest surface area to volume ratio? (virus, phagocyte, red blood cell, bacterium)

Answer 26

a phagocyte

Question 27

Are centrioles found in plants?

Answer 27

No