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Clinical Pathology > CBC Methods & RBC Analytics > Flashcards

CBC Methods & RBC Analytics Flashcards

1
Q

what color blood tube can be used for CBC

A

purple

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2
Q

what color blood tube can be used for a serum electrolyte panel

A

red

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3
Q

what color blood tube can be used for a clotting profile

A

blue

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4
Q

what color blood tube can be used for a whole blood chemistry

A

green

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5
Q

what type of anti-coagulant is in the red top tubes

A

none

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6
Q

what type of anti-coagulant is in the purple top tubes

A

EDTA

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7
Q

what type of anti-coagulant is in the blue top tubes

A

sodium citrate

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8
Q

what type of anti-coagulant is in the gray top tubes

A

oxylate

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9
Q

what type of anti-coagulant is in the green top tube

A

heparin

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10
Q

which anti-coagulants / tubes bind with calcium

A

purple (EDTA)
blue (sodium citrate)

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11
Q

what are the advantages of whole blood chemistries v. plasma or serum chems?

A

quick
no waiting for clotting

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12
Q

what 3 things are focused on a CBC

A

leukogram
erythrogram
thrombogram

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13
Q

what is the ‘most important’ part / focus of the CBC

A

erythrogram

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14
Q

how are RBCs counted in a CBC

A

impedance counting

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15
Q

what is measured with the ‘RBC’ count

A

erythrocyte concentration

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16
Q

what 3 things are used to reflect RBC mass in peripheral blood

A

Hct
Hgb
RBC

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17
Q

what is determined by the volume of RBCs

A

Hct %

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18
Q

what is the general rule to determine Hgb and Hct

A

Hgb x 3 = Hct

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19
Q

true or false:
if RBC mass in blood decreases, Hct and Hgb will increase

A

false
they all move together

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20
Q

what does pTP mean

A

plasma total protein

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21
Q

what are used in the Wintrobe RBC indices

A

MCV
MCHC
MCH

22
Q

what is MVC

A

mean cell volume

23
Q

what is the measurement used for MCV

A

average volume of RBCs

24
Q

what does MCV measure

A

each RBCs volume and averages it

25
Q

what are things that can impede the results of a MCV

A

large platelets v small RBCs
agglutinated RBCs

26
Q

what can agglutination of RBCs cause in a MCV

A

false increase of MCV

27
Q

true or false:
just because the MCV is WRI, you should look at the blood film

A

true
the sizes of the RBCs can show you macro and micro RBCs which could indicated disease

28
Q

what does MCHC measure

A

average Hgb in RBC

29
Q

when is Hgb measured

A

spectrophotometer after RBCs are lysed in patient’s tube

30
Q

what can be used to determine O2 carrying capacity

A

Hgb

31
Q

what can be used to reflect Hgb/Fe synthesis within RBCs

A

MCHC

32
Q

what does MCH measure

A

average Hgb content per RBC

33
Q

true or false
MCH is a clinically relevant indicator of anemia

A

false
although it could signal for anemia, MCH is governed by concentration of Hgb in RBCs or MCHC and not by amount of Hgb in a single erythrocyte

34
Q

why would we want to use MCHC and not MCH

A

it takes more Hgb to reach optimal concentration in larger erythrocytes

35
Q

what can usually occur due to interferents

A

factitious hyperchromic results

36
Q

what are some examples of interferents of increased MCHC

A

lipemia
hemolysis
Heinz bodies

37
Q

what is anisocytosis

A

measure of the variation in RBC size

38
Q

what is the result in RBC size with regenerative anemia

A

large cells are produced

39
Q

what is the result in RBC size with iron deficiency anemia

A

excessive numbers of small cells

40
Q

what CBC components are calculated

A

Hct
MCHC
MCH

41
Q

what CBC components are measured

A

Hgb
RBC
MCV

42
Q

what can the impact of increased MCV due to agglutination lead to

A

increased Hct

43
Q

true or false:
an increase in MCV can occur in high sodium situations

A

true

44
Q

what is Hcts

A

spun Hct

45
Q

what is Hct

A

hematocrit

46
Q

what is RBC

A

red blood cell

47
Q

what is Hgb

A

hemaglobin

48
Q

what is HCTc

A

hemacrit

49
Q

what is PCV

A

packed cell volume

50
Q

what analytes measure RBC mass

A

Hct
Hgb
RBC

Clinical Pathology (16 decks)

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