CBC Methods & RBC Analytics Flashcards
what color blood tube can be used for CBC
purple
what color blood tube can be used for a serum electrolyte panel
red
what color blood tube can be used for a clotting profile
blue
what color blood tube can be used for a whole blood chemistry
green
what type of anti-coagulant is in the red top tubes
none
what type of anti-coagulant is in the purple top tubes
EDTA
what type of anti-coagulant is in the blue top tubes
sodium citrate
what type of anti-coagulant is in the gray top tubes
oxylate
what type of anti-coagulant is in the green top tube
heparin
which anti-coagulants / tubes bind with calcium
purple (EDTA)
blue (sodium citrate)
what are the advantages of whole blood chemistries v. plasma or serum chems?
quick
no waiting for clotting
what 3 things are focused on a CBC
leukogram
erythrogram
thrombogram
what is the ‘most important’ part / focus of the CBC
erythrogram
how are RBCs counted in a CBC
impedance counting
what is measured with the ‘RBC’ count
erythrocyte concentration
what 3 things are used to reflect RBC mass in peripheral blood
Hct
Hgb
RBC
what is determined by the volume of RBCs
Hct %
what is the general rule to determine Hgb and Hct
Hgb x 3 = Hct
true or false:
if RBC mass in blood decreases, Hct and Hgb will increase
false
they all move together
what does pTP mean
plasma total protein
what are used in the Wintrobe RBC indices
MCV
MCHC
MCH
what is MVC
mean cell volume
what is the measurement used for MCV
average volume of RBCs
what does MCV measure
each RBCs volume and averages it
what are things that can impede the results of a MCV
large platelets v small RBCs
agglutinated RBCs
what can agglutination of RBCs cause in a MCV
false increase of MCV
true or false:
just because the MCV is WRI, you should look at the blood film
true
the sizes of the RBCs can show you macro and micro RBCs which could indicated disease
what does MCHC measure
average Hgb in RBC
when is Hgb measured
spectrophotometer after RBCs are lysed in patient’s tube
what can be used to determine O2 carrying capacity
Hgb
what can be used to reflect Hgb/Fe synthesis within RBCs
MCHC
what does MCH measure
average Hgb content per RBC
true or false
MCH is a clinically relevant indicator of anemia
false
although it could signal for anemia, MCH is governed by concentration of Hgb in RBCs or MCHC and not by amount of Hgb in a single erythrocyte
why would we want to use MCHC and not MCH
it takes more Hgb to reach optimal concentration in larger erythrocytes
what can usually occur due to interferents
factitious hyperchromic results
what are some examples of interferents of increased MCHC
lipemia
hemolysis
Heinz bodies
what is anisocytosis
measure of the variation in RBC size
what is the result in RBC size with regenerative anemia
large cells are produced
what is the result in RBC size with iron deficiency anemia
excessive numbers of small cells
what CBC components are calculated
Hct
MCHC
MCH
what CBC components are measured
Hgb
RBC
MCV
what can the impact of increased MCV due to agglutination lead to
increased Hct
true or false:
an increase in MCV can occur in high sodium situations
true
what is Hcts
spun Hct
what is Hct
hematocrit
what is RBC
red blood cell
what is Hgb
hemaglobin
what is HCTc
hemacrit
what is PCV
packed cell volume
what analytes measure RBC mass
Hct
Hgb
RBC
