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BMSC 210 > BMSC210 CH2.3 > Flashcards

BMSC210 CH2.3 Flashcards

1
Q

Where is the specimen placed in a brightfield microscope?

A

On a glass slide

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2
Q

What is a darkfield microscope?

A

A brightfield microscope with a modified condenser

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3
Q

What produces contrast in phase-contrast microscopy?

A

Interference of light rays

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4
Q

What does TEM stand for?

A

Transmission Electron Microscope

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5
Q

What is the total magnification if a 40 objective lens and a 10 ocular lens are used?

A

400

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6
Q

At what magnifications do maximally sharp images occur?

A

Up to about 1000

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7
Q

What does darkfield microscopy typically show?

A

Bright objects on a dark background

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8
Q

What does a darkfield microscope allow us to view?

A

Living, unstained samples of the spirochete Treponema pallidum

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9
Q

What is the wavelength of electrons that allows for better resolution than visible light?

A

0.005 nm

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10
Q

How does a TEM create an image?

A

By detecting varying opacity in different parts of the specimen

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11
Q

What is an oil immersion lens?

A

Special lens designed to be used with immersion oils

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12
Q

Why is darkfield microscopy useful for viewing live specimens?

A

Avoids the use of stains

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13
Q

What is the purpose of a phase-contrast microscope?

A

To create high-contrast, high-resolution images without staining

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14
Q

What is the purpose of a fluorescence microscope?

A

To produce an image of a specimen in bright colors against a dark background.

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15
Q

What can electron microscopy resolve?

A

Sub-cellular structures and some molecular structures

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16
Q

What do SEM images typically look like?

A

Highly detailed with a three-dimensional appearance

17
Q

What is the most commonly used type of microscope?

A

Brightfield microscope

18
Q

What are the components of a typical brightfield microscope?

A

Specimen, glass slide, stage, x-y mechanical stage knobs

19
Q

How does oil immersion improve resolution in microscopy?

A

Increases light collection and reduces light scattering

20
Q

How does a phase-contrast microscope create an image?

A

By altering the wavelengths of light rays passing through the specimen

21
Q

What is the purpose of the ocular lenses in a brightfield microscope?

A

To magnify images 10 times

22
Q

What is used to create altered wavelength paths?

A

Annular stop

BMSC 210 (24 decks)

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