Biochemical Identification of Gram Negative Bacteria (LEC/LAB) Flashcards
Gram stain reaction and morphology of Enterobacteriaceae
Gram Negative (-) Bacilli and Cocobacilli
Aerotolerance of Enterobacteriaceae
Facultative Anaerobes
Enterobacteriaceae do not produce ________.
Cytochrome C oxidase
Which member of Enterobacteriaceae produces cytochrome c oxidase?
Plesiomonas spp.
TRUE OR FALSE.
Enterobacteriaceae family produces cytochrome C oxidase.
False
Which carbohydrate/s can be fermented by the members of Enterobacteriaceae?
Glucose
Are Enterobacteriaceae motile?
Yes (at room temperature)
At which temperature are Enterobacteriaceae motile?
Room Temperature (20-25 C)
Which member/s of the Enterobacteriaceae family are not motile at room temperature?
MNEMONIC: SKY
* Shigella spp.
* Klebsiella spp.
* Yersinia spp.
Specimen of Choice for Enterobacteriaceae
Fecal/Stool Specimens
Collected within first 4 days of illness & before administration of ATBs
Specimen Collection
Fecal/stool specimens are collected within ____ of illness and before administration of antibiotics.
first 4 days
Specimen Collection
Specimens should be processed within ____ of collection.
1-2 hours
Specimen Collection
What should be done if the specimen cannot be processed within the ideal time frame (1-2 hours)?
Placed in a transport medium at the temperature of 4 C
Specimen Collection
Specimens in a transport medium must be kept at a temperature of ____.
4 C
Specimen Collection
Transport medium for Enterobacteriaceae
- Cary-Blair
- Amies
- Stuart
Which step in biochemical identification of Enterobacteriaceae is not helpful in identifying enteric pathogens?
Direct Smear Examination/Direct Microscopic Examination/Gram Staining
Which specimen sample is not helpful in direct smear preparation for identifying enteric pathogens?
Stool
Direct smear preparation of stool samples may reveal the presence of ______.
inflammatory cells
Enterobacteriaceae grow at an optimal temperature of ______.
35 - 37 C
Which organism/s is/are being described?
Grow at low temperatures (1-5 C)
- Serratia spp.
- Yersinia spp.
Serratia spp. and Yersinia spp. grow at a temperature of ______.
1 - 5 C
Which organism/s is/are being described?
Grow at high temperatures (45-50 C)
Escherichia coli
TIP: Read it as Escherichia colHIGH
Escherichia coli grows at a temperature of _____.
45 - 50 C
Colonies of Enterobacteriaceae are visible after ____ of incubation.
18 - 24 hours