Bio final sleister slides 7 (LO 17)

Question 1

Restriction enzymes

Answer 1

-Cut DNA at specific sequences called restriction sites
-Create sticky or blunt ends

Question 2

Gel electrophoresis

Answer 2

-Negatively charged DNA separate in gel based on size
-Bands can be visualized by UV light or ethidium bromide

Question 3

Ligase

Answer 3

-Joins fragments together by creating phosphodiester bonds