Bio exam 5 slides 4

Question 1

Biotechnology examples

Answer 1

-Gene therapy
-Genetic testing
-Genetically modified crops
-Biodegradable plastics

Question 2

Recombinant dna in the lab

Answer 2

-DNA isolated from cells
-Restriction enzymes cut DNA into fragments
-Gel electrophoresis allows for analysis and purification of new DNA fragments

Question 3

Restriction enzymes

Answer 3

-Cut DNA at specific sequences called restriction sites
-Produce sticky or blunt ends
-DNA ligase joints fragments together with phosphodiester bonds

Question 4

Gel electrophoresis

Answer 4

-Negatively charged DNA fragments in gel based on size
-DNA bands can be visualized with ethidium bromide and UV light

Question 5

Molecular cloning

Answer 5

-Method for producing recombinant DNA
-DNA is inserted into a plasmid vector and made into bacteria
-Plasmid vector has an origin of replication

Question 6

Expression vector

Answer 6

-Used to produce large amounts of a trans genes products

Question 7

Reproductive cloning

Answer 7

-Creates entire identical copy of organisms
-Somatic cell nuclear transfer used to replace an eggs cells haploid nucleus with diploid nucleus
-Egg develops into a zygote

Question 8

Genetic diagnosis

Answer 8

-Genetic test to determine if someone has a genetic disease

Question 9

Gene therapy

Answer 9

-Using a vector to deliver to treat/cure a disease