Bio final Sleister slides 4 (LO 10-12)

Question 1

DNA structure

Answer 1

-Right handed helix
-Each strand polymer of nucleotides
-Adjacent nucleotides linked by phosphodiester bonds
-Antiparallel- held by H bonds
-Sugar phosphate backbones
-N bases in middle
-Uniform diameter

Question 2

Leading strand

Answer 2

-Continuous synthesis towards fork
-5 to 3

Question 3

Lagging strand

Answer 3

-Discontinuous synthesis away from fork
-Uses Okazaki fragments
-5 to 3

Question 4

What enzymes used

Answer 4

-DNA polyermase III
-Ligase seals nicks in lagging strand