Bio Ch 4 Flashcards
What is a chromosome?
DNA + Histones
What is a gene? What is its purpose?
Region on the DNA that codes for protein
What percentage of our DNA contains genes?
1-2%
What are the two major components of DNA?
Sugar and phosphate
What makes up the backbone of DNA?
Deoxyribose and phosphodiester
What does it mean to say the DNA runs “antiparallel”?
It runs in opposing directions (‘5 ->3’, ‘3 ->5’)
Be able to select a complementary base pair sequence of a strand of DNA → DNA and DNA → RNA. ATTG
UAAC
What are the four nucleotides of DNA? Which one is different from RNA?
DNA: A, T, G, C
RNA: A, U, G, C
What is the name of the bond that connects the base pairs of DNA?
Hydrogen bonds
How many bonds exist between A-T? G-C?
A-T: 2
G-C: 3
What stage of the cell cycle does DNA replication occur?
“S” phase in interphase
Be able to name each stage of DNA replication, as well as the enzymes involved and their functions.
Unwind DNA: Helicase unwinds DNA
Add DNA: Primase- add RNA primer, DNA polymerase- add DNA nucleotides
Fix DNA: Ligase bind together
How is the leading strand unique from the lagging strand?
Leading: Continuous
Lagging: Discontinous
What does it mean to say the DNA is “semi-conservative”?
1 old strand, 1 new strand
What is the purpose of PCR? Gel electrophoresis?
PCR: Replicate DNA quickly
Gel electrophoresis: Identify similar DNA sequences