B2- Investigating Enzymatic Reactions Flashcards
What is the purpose of using iodine solution in the experiment?
To determine the presence of starch by changing from browny-orange to blue-black.
What temperature should the water be controlled at during the experiment?
35 °C.
What is the first step in investigating the effect of pH on amylase activity?
Put a drop of iodine solution into every well of a spotting tile.
How long should the amylase and buffer solution be incubated before adding starch?
Five minutes.
What type of solution is added to the boiling tube after the amylase and buffer solution?
Starch solution.
How often should samples be taken during the experiment?
Every 30 seconds.
What does it indicate when the iodine solution remains browny-orange?
Starch is no longer present.
Fill in the blank: To calculate the rate of reaction, you divide the amount that has changed by the _______.
time taken.
What was the time taken for amylase to break down starch at pH 6?
90 seconds.
What is the formula to calculate the rate of reaction?
Rate = change / time.
What is the rate of reaction when 24 cm³ of oxygen is produced in 50 seconds?
0.48 cm³/s.
True or False: A pH meter can be used to measure the pH of solutions in the experiment.
True.
What must be controlled to ensure a fair test in the experiment?
Concentration and volume of amylase solution.
What is the significance of using different buffer solutions in the experiment?
To see how pH affects the time taken for starch to be broken down.
