B1- More on Microscopy Flashcards
What is the first step in preparing a microscope slide for viewing onion cells?
Add a drop of water to the middle of a clean slide.
What is a microscope slide?
A strip of clear glass or plastic onto which the specimen is mounted.
What is the purpose of iodine solution when preparing a slide?
Iodine solution is a stain used to highlight objects in a cell by adding color to them.
How should you place the cover slip on the slide?
Stand the cover slip upright on the slide, next to the water droplet, and carefully tilt and lower it to cover the specimen.
What should you avoid when placing the cover slip on the slide?
Avoid getting any air bubbles under the cover slip.
What is the first action to take when using a light microscope to view your prepared slide?
Clip the slide you’ve prepared onto the stage.
What should you select before looking through the eyepiece of a light microscope?
Select the lowest-powered objective lens.
What is the function of the coarse adjustment knob?
To move the stage up or down to focus the image.
What should you do if you need to see the slide with greater magnification?
Swap to a higher-powered objective lens and refocus.
What should your drawing of the specimen include?
A title of what you were observing and the magnification under which it was observed.
What is the formula to calculate magnification?
Magnification = length of drawing of cell / real length of cell.
What should your drawing of cells not include?
Any coloring or shading.
What is the recommended drawing tool for observations under a microscope?
A pencil with a sharp point.
True or False: The subcellular structures in your drawing should be drawn in proportion.
True.
What should you use to label important features of your drawing?
Straight, uncrossed lines.
