b/b section bank Flashcards
Kd
Dissociation constant/concentration of ligand. As Kd decreases, affinity increases.
Kd = ([L][R]) / [LR]
peptide hormones
hydrophilic, soluble in blood.
hormones that bind to transport proteins
steroid proteins, lipophilic
bonds that contribute to teritary structure of a protein
disulfide, hydrogen, salt bridge…
why doesnt uncompetitive alter the Km/Vmax of an enzyme?
Because both are reduced, there is no change in slope
nuclear factors
the only elements that can vary in different cells and can confer both temporal and spatial regulation of their target genes.
what type of amino acid are most likely to dimerize?
hydrophobic amino acids, because hydrophillic amino acids or polar amino acids are more likely to interact with water rather than each other.
approximate weight of amino acids
110 Da
how many reduced electron carriers are generated during conversion of keotglutarate to oxaloacetate
three: 2 molecules of NADH and one molecule of FADH2
which enzyme catalyzes the final steps of gluconeogenesis and glycogenolysis
G6P
Glucose 6-phosphatase
Efficiency of an enzyme
Kcat / Km
Vmax = Kcat[E]
enzymes involved in dna cloning
dna polymerase, dna ligase, RT
increase in Kd indicates
a higher dissociation constant , less stability.
pi stacking
noncovalent attractive forces between two aromatic rings.
G protein coupled receptor binding
Glucagon binds, activates GPCR.
GDP dissociates, GTP binds.
Adeylate cyclase, PKA and cAMP increase.
