Analisis Tanaman Transgenik 2 Flashcards

1
Q

Transgen berupa

A

Gen hpt dan gen MmCu/Zn-SOD

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2
Q
  1. Hygromycine berperan
  2. Hygromycine B dihasilkan oleh
A
  1. Menghambat sintesis polipeptida dengan menstabilisasi situs akseptor tRNA pada ribosom sehingga menghambat translasi baik pada prokariot maupun eukariot
  2. Streptomyces hugroscopius
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3
Q
  1. Pada E. coli dihasilkan
  2. Berfungsi untuk
A
  1. hpt, hph/aphIV
  2. Mendetoksikasi aminocyclitol antibiotic hyromycin B
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4
Q

hpt-MmCu/Zn-SOD
1. Gen hpt terpaut sempurna dengan (r=…) dan diwariskan bersama sama
2. Pada tembakau, pada tomat
3. rH-S=

A
  1. Gen MmCu/Zn-SOD (r= 0 cM)
  2. 1 cM~1250kb, 1 cM~750 kb
  3. (r1+r2)/total
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5
Q

A. tumefaciens strain … mengandung pGWB502-MmCu/Zn-SOD

A

LBA4404

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6
Q

Tanaman transgenik, To

A

Seragam heterozigot

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7
Q

Merakit galur murni transgenik

A
  1. Transgenik breeding line
  2. Homozigot transgenik
  3. Introgresi ke galur lain
  4. Uji tantang (bioassay)
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8
Q

Homozigitas tanaman transgenik
1. Tanaman resisten H
2. Populasi keturunan diseleksi terhadap
3. Jika populasi keturunan semuanya resisten
4. Jika populai keturunan ada yang sensitif

A
  1. menyerbuk sendiri
  2. higromisin
  3. Tetua homozigot
  4. Tetua heterozigot
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