A2.2 (part 1) Flashcards
light microscope magnification
10 to 400 x
only used for living specimens
spontaneous generation theory
organisms appear from non living matter, provn wrong by Hocks, by his seeing cells com uma microscope
coverslip for microscope
place on 90 degreese angle
stadge micrometer
ruler attatched to stadge
eye piece graticule
grid on to measure things in microscopes
iodine stains used for
plants
methylene blue stain used for
animals
gram staining
for bacteria, has + and -
1mm is
m\ 1000 is mm\ 1000 is micrometers \ 1000 is nm
electron microscope
black and white
dead speciminens
how electron microscopes work
they shine beam of electrons through and they will pass through less dense areas but wil gt absorbed to dense ones (electrons have a shorter wave lenght than light so pass through
freeze fracture microscopy technique
freeze sample
break
observe com electron
can observe things which normally are not visible, like intenal plasma membranes (mostly for membranes)
cryogenic electron microscope
freeze to -180 degreese or colder which stops molcules from moving
this impvoes resolution and prevents electron beam damage
immuo fluorescence microscope
better viualiation of sructures
fluro tag attatches to antigens and this will be shonwn as ight of diffrent colours
for study of diseases
(for light eu pensa)
fluroscent dye
light microsopy
dye atttches to prefered substance, and will appear coloued
visualises drug delivery