A&P Lab Practical 2 Flashcards
Ocular lens
eyepiece / magnifies the image 10X
Objective lens
further magnifies the image
Nosepiece
holds the objectives and can be turned to change objectives
Arm
supports the body tube
Light source
provides light for viewing the image
Stage
supports a slide. A mechanical stage is movable.
Mechanical stage knobs
allow the movement of the mechanical stage.
Slide holder
secures the slide in position for viewing
Iris diaphragm
lever located under the stage. Controls the amount of light that is allowed through the object being viewed.
Coarse focus knob
moves the body tube up and down in large increments to allow gross positionin and focusing.
Fine focus knob
moves the body slightly to bring the image into sharp focus
Base
supports the microscope
Magnification
of times larger than itself an object appears to be. Image may not be clear.
Resolution
ability to distinguish two objects as separate entities.
Fine & Coarse focus knobs are responsible for allowing the _____ of a specimen.
resolution
The ocular and objective lenses are responsible for the ______ of a specimen.
magnification
What is the magnification of the Ocular Lens?
10X
What is magnification of the following objective lenses?
- Scanning
- Low Power
- High Power (dry)
- Scanning——–4X
- Low Power —–10X
- High Power (dry-40X
Because you use both the ocular and objective lens, the specimen is magnified twice.
Calculate the Total Magnification when the Objective lens is the following:
Low Power.
Ocular lens x Objective lens = Total Magnification
Low Power. 10X x 10X = 100X
Because you use both the ocular and objective lens, the specimen is magnified twice.
Calculate the Total Magnification when the Objective lens is the following:
High Power (dry).
Ocular lens x Objective lens = Total Magnification
High Power (dry). 10X x 40X = 400X
Because you use both the ocular and objective lens, the specimen is magnified twice.
Calculate the Total Magnification when the Objective lens is the following:
Scanning.
Ocular lens x Objective lens = Total Magnification
Scanning. 10X x 4X = 40X
What is the calculation for total magnification?
Total magnification = Magnifcation of Ocular lens x Magnification of Objective lens
Working distance
the distance from the objective lens to the specimen being observed
The benefit of ____ power lenses is that they _____ the images and allow you to see more _____.
The drawback is that the ____ power lens you use, the ___ the area of the specimen you see.
This is because the ___ power lenses have smaller ________.
high / enlarge / detail
higher / smaller
high / openings (apertures)
Working distance ______ considerably when you move from ____ to ___ power.
decreases
low
high
Field of view
the circular view seen in the eyepiece
Using the Microscope Procedure.
Use the ___________ to raise the stage towards the _________ as far as possible.
Watch from the ____ to make sure the _____ does not touch the slide.
coarse focus knob / scanning (4x) objective lens
side / objective lens
Using the Microscope Procedure.
Adjust the light by manipulating the ____ and ______ until the hole (______) provides the best light for observation.
light source / diaphragm / aperture
Never use the ______ focus knob when the low power or high dry power objective lens is in place.
coarse
Explain.
Parfocal
Parfocal means little refocusing is required when moving from one lens to another.
Using the Microscope Procedure.
After you use scanning lens to center and focus on a specimen, and then switch to the low power lens.
The specimen should still be ________ because the microscope is ________.
in focus
parfocal
True or False.
To fully appreciate the details of a specimen you should constantly be changing the depth of focus with the fine adjustment knob.
True
Using the Microscope Procedure.
The _____ depth perceived when looking through a microscope is called the ______.
It is _____ to see all parts of all specimens clearly at any one focal plane.
3-D / depth of field
impossible
ID the ocular, fine and coarse adjustment knobs.
Adipose tissue
Compact Bone
Hyaline cartilage
Simple Columnar Epithelium
Stratified Squamous Epithelium
Identify the major function.
Cell membrane
- Physical barrier between intracellular and extracellular environment.
- Regulates exchange of nutrients, waste, etc.
- Monitors changes in the surroundings.
- Structural support by connecting with other cells.
Identify the major function.
Nucleus
- Stores and processes genetic information.
- Assembles ribosomes.
Identify the cellular component.
The outer, non rigid boundary of the cytoplasm.
Cell membrane
Identify the cellular component.
The lightly stained portion of the cell, outside the nucleus.
Cytoplasm
Identify the cellular component.
A spherical, stained body located in the central portion of the cell.
Nucleus
Identify the cellular component.
A tiny, darker-stained body within the nucleus.
Nucleolus