9. cell fractination

Question 1

Whats is cell fractionation? Why is it done?

Answer 1

This is the process used to separate cellular components while preserving the function of each component. It is done to study the organelles.

Question 2

How do we separate the cellular components? What’s it the most dense organelle and least dense organelle?

Answer 2

It can be done with centrifugation where the organelles are separated based on density.

The most dense organelle is the nuclei the least dense organelle is the ribosome.

Question 3

What else can you do to separate it?

Answer 3

You can use homogenisation. It uses a mix of solution that is ground and minced and chopped with the sample tissue to stop osmotic damage