7. Immunohistochemistry

Question 1

What is imunohistochemistry?

Answer 1

Immuohistochemistry is the process of using Antibodies to identify the presence of certain antigens.

Question 2

Describe direct imunohistochemistry?

Answer 2

It’s when the antigen of interest is directly bonded to a primary antibody, specific for that antigen.

Question 3

Describe indirect immunohistochemistry?

Answer 3

Indirect imunohistochemistry uses an unlabelled primary antibody which is only detected when bound to secondary antibodies of that antigen.

Question 4

What’s an polyclonal antibody? What is an monoclonal antibody?

Answer 4

A polyclonal antibody means that it arisen from a different B-cell within the body.

Monoclonal is a clone of one unique parent cell.

Question 5

Why would imunohistochemistry be usefull?

Answer 5

These methods are used widely for detection of abnormal cells such as cancerous tumours.