4. Histochemistry. Methods Of Demonstration Of Protein,lipids And Carbs

Question 1

How can polysaccharides(Carbohydrates) be demonstrated? What happens in this reaction? What colour does it get?

Answer 1

It can be demonstrated by periodic acid schiff(PAS) reaction.

The reaction breaks up the bonds between two adjacent carbons in the polysaccharide allowing the reaction to give it purple colour.

Question 2

How can lipids be demonstrated?

Answer 2

It can be demonstrated by Sudan 3 och Sudan black.

Question 3

How can proteins be demonstrated? Amino acids? Weigerts resorcinol?

Answer 3

Protein can be demonstrated in many different ways depending on the type of protein. Amin acids can be demonstrated by imunocytochemistry. Weigerts resorcin can be demonstrated by fucsin.