6.1 Flashcards
culturing
growing a large number of microrgansims
provide with the right level of nutrients ph and temp
aseptic techniques
important because stops unwanted bacteria which is good for safety and results
inoculation
getting the bacteria onto your agar
can be done using an inoculation loop
how to grow pure culture
anarobic conditions- only some can survive
nutrient requirements
indicator media
cell counting
use a microscope and hemocytometer
-slide with grids
use the turbidity- more turbid more cells
- use colorimeter
dilution plating
-1 in 10 or 1 in 2
-use maths
area and mass of fungi
generation time
time between divisions
can use logarirthmic scale
expontential growth rate
bacterial growth graph stages
lag- bacteria adapting to new enviroment
log- rate is close to or at maximum
stationary- total rate is 0 cells made is equal to cells dying
death- death rate is increasing and growth is close to 0
why does growth decrease
nutrients available getting used up
build up of waste products
endotoxins
lipopolysaccharides
outerlayer of the cell wall of gram negative bacteria
effect on the site of the infection as restricted to the wall
salmonella
exotoxins
soluble proteins
free to move around
act as enzymes to break down
act as enzymes inhibitors to prevent normal enzyme function
bind to proteins
stapyloccus
bacteriostatic
completly inhibits the growth of the microorganism
bactericidal
destroy almost all of the pathogens present
used for severe infections
factors of antibiotics
concentration
local ph
whether the pathogen or the host tissue destroy
susceptibility of the pathogen
