5.5) Antibodies Flashcards
What is the role of the disulfide bridge in forming the quaternary structure
of an antibody?
It join two polypeptides
What are antibodies?
They are proteins synthesised by B cells
What is formed when antibodies attach to antigens
They form an antigen-antibody complex
What is the structure of antibodies?
They are made up of forming 4 different polypeptide chains
What is the binding site on antibodies known as?
The variable region
What is the remaining of the antibody called?
A constant region
What is a monoclonal antibody ?
It’s antibodies that are isolated produced by a single clone of cells
–> Antibodies with the same tertiary structure
What are some of the uses of monoclonal antibodies?
- Cancer Treatment
- Transplant surgery
*Immunoassay ( method of calculating the amount of substance in a mixture)
What is the role of the antibodies in the ELISA test
- Antibody attaches to antigen (complimentary to antigen in shape)
- Antibody with enzyme attached is added;
- Antibody attaches to antigen;
- The second antibody attaches to first antibody
- Substrate/solution added and colour changes
Describe and explain the role of antibodies in stimulating phagocytosis.
- Antibodies bind to antigen
- Antibodies cause clumping
