3.3.1: Enzymes & digestion Flashcards
what is mechanical digestion and why does it happen
teeth break down large pieces into smaller pieces - to increase surface area for digestion and so increase rate of digestion
What is chemical digestion
involves enzymes, hydrolysing of bonds - glycosidic, peptide, ester - breakdown of large insoluble molecules to small soluble ones
explain why it is necessary for food to be digested in order to be absorbed into our body
to make it smaller and soluble so that it can be transported across membranes to be absorbed into the blood plasma
list 3 functions of mucus in the digestive system
1 - neutralise acid
2 - provides lubrication
3 - protects gut wall from acid erosion
state where enzymes and digestive chemicals are secreted from and where they go, as part of extracellular digestion
enzymes are secreted from:
salivary glands into mouth
gastric glands into stomach
pancreas into duodenum
digestive chemicals are secreted from pancreas into duodenum
state where intracellular digestion happens and what happens in the process
occurs in the epithelial cells of ileum, they hydrolyse disaccharides and dipeptides
describe the process of carbohydrate digestion (starch)
amylase hydrolyses starch to make maltose which is then hydrolysed by maltase to make glucose
describe what the role of amylase is, where it is found and its optimum pH
hydrolyses glycosidic bonds in starch to produce maltose. Amylase is found in saliva and pancreatic juice, its optimum pH is 8
describe what the role of maltase is, where it is found and its optimum pH
hydrolyses glycosidic bonds in maltose to produce glucose. Maltase is found in epithelial cells in ileum and its optimum pH is 8
describe and explain the process of protein digestion
pepsin (endopeptidase) in the stomach hydrolyses peptide bonds in protein into polypeptide chains
trypsin (endopeptidase) in the duodenum hydrolyses the polypeptide chains into smaller polypeptide chains. Exopeptidases in the duodenum remove the amine group from the polypeptide chain to produce amino acids and dipeptides (by hydrolysis)
Dipeptidases (exopeptidases) in the ileum hydrolyse the dipeptides into amino acids
what is the difference between exopeptidases and endopeptidases
exo = hydrolyse the end of the polypeptide chain
endo = hydrolyse the inside of the polypeptide chain
name the 2 endopeptidases involved in protein digestion and state where they are found, their optimum pH
pepsin - stomach pH 2
trypsin - duodenum pH 8
state where exopeptidases are found and give 3 examples
found in pancreas and epithelial cells of ileum: 1 - carboxypeptidase
2 - aminopeptidase
3 - dipeptidase
explain why it is more efficient for the endopeptidase enzymes to act before the exopeptidases
the endopeptidases hydrolyse the internal peptide bonds in the chain first which increases the surface area for exopeptidases to hydrolyse the external peptide bonds
Describe and explain the process of lipid digestion
process of mechanical digestion happens first: bile salts emulsify lipids into micelles, this increases surface area for lipase action. Afterwards, process of chemical digestion happens: lipase hydrolyses ester bonds in the micelles into monoglycerides and fatty acids
where is bile produced and stored, and how is it released and where to
bile is produced in the liver, stored in the gallbladder, released via the bile duct to the duodenum
what two things does bile contain
1 - bile salts that emulsify lipids into micelles
2 - sodium hydrogen carbonate that neutralises stomach
name the substrate that amylase hydrolyses and the product made
starch -> maltose